Optical Doppler tomography of human skin

描述（由申请人提供）： 该项目的长期目标是开发一种非侵入性光学技术，用于以高空间分辨率成像人体皮肤中的血流。光学多普勒层析成像（ODT）将多普勒测速与光学相干层析成像（OCT）相结合，以测量高度散射的生物组织中用户指定的离散位置处的血流速度。ODT的极高分辨率允许对体内血液微循环和血管周围的组织结构进行无创成像。我们在这项研究中的中心假设是，在激光治疗之前，ODT提供了一种实用的方法来表征葡萄酒色斑（PWS）胎记。为此，我们提出了四个具体目标：（1）设计并构建基于光谱干涉的光学检测系统;（2）开发成像构建算法;（3）利用高速光学检测系统进行一系列的活体血流成像实验，以验证图像的准确性和保真度;（4）建立一套基于光谱干涉的光学检测系统。以及（4）在临床研究中证明ODT如何能够帮助接受激光治疗的PWS患者的临床管理。在对体外混浊样品、体内模型血管和原位PWS人类皮肤进行的初步研究中，已经证明了ODT在高度散射的生物组织（例如人类皮肤）中的离散用户指定位置以高空间分辨率表征和成像血流的可行性和潜在应用。本文提出的研究的目的是扩展这些初步研究结果，并有望导致PWS患者临床管理的新技术的开发。

DESCRIPTION (provided by applicant): The broad, long-term objective of this project is the development of a non-invasive optical technique for imaging blood flow in human skin with high spatial resolution. Optical Doppler Tomography (ODT) combines Doppler velocimetry with optical coherence tomography (OCT) to measure blood flow velocity at discrete user-specified locations in highly scattering biological tissues. The exceptionally high resolution of ODT allows noninvasive imaging of both in vivo blood microcirculation and tissue structures surrounding the vessel. Our central hypothesis in this research proposal is that ODT provides a practical methodology for characterization of port wine stain (PWS) birthmarks, prior to institution of laser therapy. To this end, we propose four specific aims: (1) an ODT system based on spectral interferometry will be designed and constructed; (2) an imaging construction algorithm will be developed; (3) a series of experiments to image in vivo blood flow will be carried out using the high speed ODT system to confirm image accuracy and fidelity; and (4) demonstrate in clinical studies how ODT can assist in the clinical management of PWS patients receiving laser therapy. In preliminary studies conducted on in vitro turbid samples, in vivo model vasculatures and in situ PWS human skin, the feasibility and potential application of ODT to characterize and image blood flow with high spatial resolution at discrete user-specified locations in highly scattering biological tissues such as human skin has been demonstrated. The objective of the studies proposed herein is to extend these initial findings and is expected to lead to the development of a new technology for the clinical management of PWS patients.