Nanoscale Electrocatalytic Protein Detection

描述(由申请人提供)： 这项建议集中在一个新的系统，用于电化学检测癌症相关的蛋白质靶标使用纳米级的电极平台。提出的分析依赖于涉及两个过渡金属离子的电催化过程，该过程报告了生物分子络合事件。由于报告系统对电极表面静电的变化做出反应，它将使核酸/蛋白质和多肽/蛋白质复合体的分析成为可能。该项目有3个具体目标：1)使用DNA修饰电极对与癌症有关的DNA修复蛋白进行电催化检测；2)优化电催化检测和细胞DNA修复活性的多重分析；3)发展一种使用前列腺癌生物标志物的通用电催化蛋白检测方法。所描述的电催化蛋白质检测系统是有利的，因为它将允许分析和识别除非络合分析物之外的多蛋白质复合体，并且将具有高的选择性和特异性。此外，蛋白质检测分析将使用纳米电极进行，这将允许对不同生物分子靶标的面板进行多路复用检测。

DESCRIPTION (provided by applicant): This proposal focuses on a novel system for the electrochemical detection of cancer related protein targets using a nanoscale electrode platform. The assay proposed relies on an electro catalytic process involving two transition-metal ions that reports on biomolecular complexation events. Because the reporter system responds to changes in the electrostatics of an electrode surface, it will enable the analysis of nucleic acids/protein and peptide/protein complexes. The proposed project has 3 specific aims: 1) electro catalytic detection of DNA repair proteins implicated in cancer using DNA-modified electrodes; 2) optimization of electro catalytic detection and multiplexed analysis of cellular DNA repair activities, and 3) development of a generalized electro catalytic protein detection method using prostate cancer biomarkers. The electro catalytic protein detection system described is advantageous because it will allow the analysis and discrimination of multiprotein complexes in addition to uncomplexed analytes and will have high selectivity and specificity. Additionally, the protein detection assays will be conducted using nanoelectrodes that will allow multiplexed detection of panels of different biomolecular targets.