Nanoscale Electrocatalytic Protein Detection

描述（由申请人提供）： 该提案的重点是使用纳米级电极平台电化学检测癌症相关蛋白质靶点的新系统。建议的测定依赖于涉及两个过渡金属离子的电催化过程，该过程报告生物分子络合事件。由于报告系统响应电极表面的静电变化，因此它将能够分析核酸/蛋白质和肽/蛋白质复合物。拟议的项目有3个具体目标：1）使用DNA修饰电极电催化检测与癌症有关的DNA修复蛋白; 2）优化电催化检测和细胞DNA修复活动的多重分析，以及3）开发使用前列腺癌生物标志物的通用电催化蛋白检测方法。所描述的电催化蛋白质检测系统是有利的，因为除了未复合的分析物之外，它还允许分析和区分多蛋白质复合物，并且具有高选择性和特异性。此外，蛋白质检测试验将使用纳米电极进行，这将允许对不同生物分子靶标的组进行多重检测。

DESCRIPTION (provided by applicant): This proposal focuses on a novel system for the electrochemical detection of cancer related protein targets using a nanoscale electrode platform. The assay proposed relies on an electro catalytic process involving two transition-metal ions that reports on biomolecular complexation events. Because the reporter system responds to changes in the electrostatics of an electrode surface, it will enable the analysis of nucleic acids/protein and peptide/protein complexes. The proposed project has 3 specific aims: 1) electro catalytic detection of DNA repair proteins implicated in cancer using DNA-modified electrodes; 2) optimization of electro catalytic detection and multiplexed analysis of cellular DNA repair activities, and 3) development of a generalized electro catalytic protein detection method using prostate cancer biomarkers. The electro catalytic protein detection system described is advantageous because it will allow the analysis and discrimination of multiprotein complexes in addition to uncomplexed analytes and will have high selectivity and specificity. Additionally, the protein detection assays will be conducted using nanoelectrodes that will allow multiplexed detection of panels of different biomolecular targets.