Development of DNA-templated IR quantum dots

DNA 模板红外量子点的开发

• 批准号: 7368599
• 负责人: Shana O Kelley
• 金额: $ 12.96万
• 依托单位: UNIVERSITY OF TORONTO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7368599
• 关键词: Address; Binding; Biological; Biological Assay; Biological Markers; Biology; Biosensor; Caliber; Cells; Cellular Assay; Chemicals; Chemistry; Class; Compatible; Coupling; Cultured Cells; DNA; DNA Sequence; Detection; Development; Diagnosis; Diagnostic Imaging; Disease; Engineering; Environment; Exhibits; Generations; Glutamate Carboxypeptidase II; Growth; In Vitro; Kidney; Libraries; Life; Malignant Neoplasms; Medical; Medicine; Methods; Modification; Monitor; Nucleic Acids; Nucleotides; Oligonucleotides; Optics; Organic solvent product; Organism; Penetration; Performance; Plasma; Production; Property; Prostate-Specific Antigen; Quantum Dots; Range; Reporting; Research Infrastructure; Resources; Route; Semiconductors; Solutions; Specificity; Staging; Structure of molecular layer of cerebellar cortex; Surface; Techniques; Time; Today; Toxic effect; Vertebral column; aptamer; aqueous; base; cancer diagnosis; cellular imaging; combinatorial; design; fluorophore; improved; in vivo; luminescence; nanocrystal; nanomaterials; nanoparticle; nanoscale; novel; novel strategies; particle; programs; quantum; size; tumor

DESCRIPTION (provided by applicant): Functionalized semiconductor quantum dots have previously been demonstrated to bind to markers on cells. Through their spectrally-narrow optical emissions, they illuminate tumors and other harbingers of disease. They are enabling the highly specific detection of a range of diseases at the earliest stages. We propose to pursue a new and improved class of semiconductor quantum dots. We have shown already that, by seeding the growth of nanoparticles using a DNA template, we are able to produce quantum dots that are efficient, stable emitters in the infrared wavelengths (so-called biological window) in which living organisms are much more transparent than in the visible wavelengths, and in which living organisms' autofluorescence is orders of magnitude lower than in the visible. These materials retain their luminescence properties over time even in biological media such as plasma at 37o C. The collaborating team of Dr. Shana Kelley, a nucleic acids chemist, and Dr. Edward Sargent, an optoelectronics engineer, bring the expertise required to optimize the DNA-grown nanoparticles for applications in medical diagnosis. The strategy of directing the growth of luminescent nanoparticles using DNA provides a one-pot route towards the strong coupling of light-emitting tags with DNA-based aptamers for programmable specific-binding. The project is divided into the following Specific Aims: 1) Direct the growth, and thereby maximize the performance, of DNA-templated quantum dots using designer DNA sequences; 2) Discover optimized DNA sequences for specific binding assays using in vitro selection. We will thereby develop new means of creating highly luminescent nanomaterials for medicine and biology. The team, with its complementary expertise in biomolecular chemistry and optical materials, is equipped with the infrastructure and resources to make a significant contribution to the realization of improved visible and infrared fluorophores for diagnosis.

描述（由申请人提供）：官能化半导体量子点先前已被证明与细胞上的标记物结合。通过其光谱狭窄的光发射，它们照亮肿瘤和其他疾病的先兆。它们使得能够在早期阶段高度特异性地检测一系列疾病。 我们建议追求一种新的和改进的半导体量子点。我们已经证明，通过使用DNA模板接种纳米颗粒的生长，我们能够产生在红外波长（所谓的生物窗口）中有效，稳定的发射器的量子点，其中生物有机体比可见光波长透明得多，并且其中生物有机体的自发荧光比可见光低几个数量级。这些材料即使在37 ° C的等离子体等生物介质中也能保持其发光特性。核酸化学家Shana Kelley博士和光电子工程师Edward萨金特博士的合作团队带来了优化DNA生长纳米颗粒用于医疗诊断所需的专业知识。 使用DNA引导发光纳米颗粒生长的策略提供了一种一锅法路线，该路线朝向发光标签与基于DNA的适体的强耦合，用于可编程的特异性结合。该项目分为以下具体目标：1）使用设计的DNA序列指导DNA模板量子点的生长，从而最大限度地提高性能; 2）使用体外选择发现用于特异性结合测定的优化DNA序列。因此，我们将开发新的方法，为医学和生物学创造高度发光的纳米材料。该团队在生物分子化学和光学材料方面具有互补的专业知识，配备了基础设施和资源，为实现改进的可见光和红外荧光团诊断做出了重大贡献。