Detection of H. pylori using electrical DNA sensing

使用电 DNA 传感检测幽门螺杆菌

• 批准号: 6622762
• 负责人: Shana O Kelley
• 金额: $ 22.79万
• 依托单位: BOSTON COLLEGE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6622762
• 关键词: Helicobacter; bacterial DNA; biopsy; biosensor device; biotechnology; clinical research; electrical conductance; electrical measurement; gastrointestinal disorder diagnosis; gastrointestinal infection; genetic techniques; human subject; nanotechnology; patient oriented research; technology /technique development

DESCRIPTION (provided by applicant): This proposal describes the development of a new class of biosensors exploiting an intrinsic property of DNA: electrical conduction. The capability of dsDNA to conduct electricity, in combination with the ability to measure this conduction in short (<100bp) and specific DNA sequences, will be harnessed to produce sensors with unprecedented sensitivity and portability. Conventional methods of detecting sequences on DNA arrays employ optical detection, and lack the sensitivity required to detect microbial species in clinical samples without extensive processing. The goal of this project is to construct a nanoscale DNA sensor with electrical detection that can sensitively and accurately detect Helicobacter pylori, a bacterium associated with gastric ulcers and cancer, in clinical samples. The specific aims of this project include (1) the demonstration of proof-of-principle for electrical DNA sensing carried out with synthetic DNA molecules modeling a unique H. pylori gene, and (2) the detection of H. pylori in clinical samples using electrical DNA detection.

描述(由申请人提供)：本建议书描述开发情况 一种利用DNA固有特性的新型生物传感器： 导电性。DsDNA的导电能力，在 与短时间内测量此传导的能力(&lt；100个基点)和 特定的DNA序列，将被利用来制造具有 前所未有的敏感度和便携性。传统的检测方法 DNA阵列上的序列采用光学检测，缺乏敏感性 要求检测临床样本中的微生物种类，而不需要广泛的 正在处理。这个项目的目标是构建一个纳米尺度的DNA传感器。 具有电检测，可以灵敏而准确地检测到 幽门螺杆菌，一种与胃溃疡和癌症有关的细菌，在 临床样本。该项目的具体目标包括：(1) 开展了DNA电传感的原理验证 用合成的DNA分子模拟独特的幽门螺杆菌基因，以及(2) DNA电泳法检测临床标本中幽门螺杆菌