Genotypes of Helicobacter Pylori in West Africa

西非幽门螺杆菌的基因型

• 批准号: 6933857
• 负责人: RICHARD A ADEGBOLA
• 金额: $ 5.4万
• 依托单位: MEDICAL RESEARCH COUNCIL UNIT THE GAMBIA AT THE LONDON SCHOOL OF HYGIENE & TROPICAL MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933857
• 关键词: Africa; Helicobacter; acidity /alkalinity; adult human (21+); age difference; bacterial genetics; biochemical evolution; children; chronic disease /disorder; clinical research; communicable disease transmission; disease /disorder onset; disease /disorder proneness /risk; drug resistance; gastroscopy; gene environment interaction; genetic polymorphism; genetic strain; genotype; host organism interaction; human subject; metronidazole; microorganism classification; microorganism culture; microorganism population study; tube feeding

DESCRIPTION (provided by applicant): Helicobacter pylori infects over half of adults worldwide. Colonization begins in childhood, and contributes to gastroduodenal disease in adult life. Strains obtained close to colonization are rarely encountered, as they require isolation from young children, yet a detailed study of such strains is critical to understanding H. pylori associated disease. Our study will be conducted in The Gambia, W. Africa, the ancestral home of many African Americans, in a population with a high early childhood incidence of H. pylori colonization. The sadly frequent need for the use of naso-gastric tubes to intensively re-feed malnourished infants, and the high early childhood incidence of H. pylori infection, allow us a unique opportunity to investigate colonizing strains of H. pylori, and further develop appropriate local approaches to disease management. Our goal is to evaluate new methods for culturing H. pylori strains from young children, and to compare these to those obtained from adults. We propose that the transition from childhood colonization to adult disease state is affected by multiple factors including evolution of genotypes of colonizing H. pylori strains themselves, so that H. pylori strains from Gambian children will differ genetically from strains from chronically colonized adults. We have two specific aims: Aim 1: To compare the range of genotypes obtained from H. pylori cultured from gastric biopsies from Gambians, with those obtained by culture of gastric juice aspirate, from the same subjects: Aim 2: To assess the relatedness of child strains and those from close adult relatives, cultured from naso-gastric aspirates. The data from these experiments will give us our first insight into the changes that may occur in the H. pylori genome during the period of colonization of its unique niche, the human stomach. We anticipate that our work will allow further understanding of why the clinical diseases associated with chronic H. pylori colonization in West Africa differ from those seen in industrialized nations, and enable the development of appropriate treatment strategies for communities such as The Gambia.

描述（由申请人提供）：幽门螺杆菌在全球范围内感染了一半以上的成年人。殖民化始于童年，并在成人生活中促成胃二维亚疾病。由于需要与幼儿隔离，因此很少遇到接近定植的菌株，但对这种菌株的详细研究对于理解幽门螺杆菌相关疾病至关重要。我们的研究将在许多非洲裔美国人的祖先的冈比亚W. Africa进行，幽门螺杆菌殖民地的早期发病率很高。可悲的是，频繁地使用鼻gastric管来强烈地重新喂养营养不良的婴儿，以及幽门螺杆菌感染的幼儿幼儿发病率，这使我们有一个独特的机会来研究幽门螺杆菌的定植菌株，并进一步开发出适当的局部方法来治疗疾病的局部方法。 我们的目标是评估从幼儿培养幽门螺杆菌菌株的新方法，并将这些方法与从成年人获得的方法进行比较。我们建议，从童年定殖到成人疾病状态的过渡受多种因素的影响，包括幽门螺杆菌菌株的基因型的进化，因此，冈比亚儿童的幽门螺杆菌菌株在遗传上与长期殖民成年人的遗传菌株有所不同。我们有两个具体的目的：目标1：比较从冈比亚人的胃活检中培养的幽门螺杆菌获得的基因型范围，以及通过同一主题的胃果汁培养物获得的基因型：目标2：评估儿童菌株的相关性和来自近距离的成人亲戚的相关性，从鼻孔中培养的naso-gastric Asperates。这些实验的数据将使我们对幽门螺杆菌基因组在其独特的小裂胃中可能发生的变化中可能发生的变化。我们预计，我们的工作将进一步了解为什么西非与慢性幽门螺杆菌殖民相关的临床疾病与工业化国家中的临床疾病不同，并能够为冈比亚等社区制定适当的治疗策略。

项目成果

Mixed infection with cagA positive and cagA negative strains of Helicobacter pylori lowers disease burden in The Gambia.

• DOI: 10.1371/journal.pone.0027954
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Secka O;Antonio M;Berg DE;Tapgun M;Bottomley C;Thomas V;Walton R;Corrah T;Thomas JE;Adegbola RA
• 通讯作者: Adegbola RA

Population genetic analyses of Helicobacter pylori isolates from Gambian adults and children.

冈比亚成人和儿童幽门螺杆菌分离株的群体遗传学分析。

• DOI: 10.1371/journal.pone.0109466
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Secka,Ousman;Moodley,Yoshan;Antonio,Martin;Berg,DouglasE;Tapgun,Mary;Walton,Robert;Worwui,Archibald;Thomas,Vivat;Corrah,Tumani;Thomas,JulianE;Adegbola,RichardA
• 通讯作者: Adegbola,RichardA