CORE--ASSAY

该核心测量血清、血清超滤液和尿液化学，计算人类(由Coe和Lingeman项目)和猪(由Evan项目)的尿液过饱和度(SS)，以及执行血清草酸的分析(项目Core1)。该核心还执行测试，以测量尿液中草酸钙和磷酸钙的亚稳上限(由Coe和Lingeman项目)。核心测量用于计算硫代氨基甲酸盐清除量的血清和尿液中的硫代氨基甲酸(COE项目)。计算正常血糖-高胰岛素葡萄糖钳夹诱导的葡萄糖利用(项目 由COE)与糖尿病研究和培训中心合作。外周血单核细胞维生素D受体水平(PBM VDR)将在建议的GCRC方案(由COE项目)研究的患者中进行测定。从Lingeman项目获得的肾皮质活检标本中将进行VDR、钙受体(CAR)、calbindin 9和calbindin 28的蛋白质丰度分析(通过Western印迹)；VDR和CAR的mRNA表达水平将被测量。

This core measures serum, serum ultrafiltrate, and urine chemistries and calculates urine supersaturations (SS) for human (Projects by Coe and Lingeman) and pig {Project by Evan) protocols, as well as performing assays of serum oxalate (Project core1). The core also performs assays to measure upper limit of metastability with respect to calcium oxalate and calcium phosphate in urine (Projects by Coe and Lingeman). The core measures iothalamate in serum and urine for calculation of iothalamate clearances (Project by Coe). Calculation of euglycemic-hyperinsulinemic glucose clamp induced glucose utilization will be done (Project by Coe) in collaboration with the Diabetes Research and Training Center. Peripheral blood monocyte vitamin D receptor levels (PBM VDR) will be determined in patients studied in the proposed GCRC protocols (Project by Coe). Assays of protein abundance (by Western blot)will be carried out for VDR, calcium receptor (CaR), calbindin 9 and calbindin 28 in renal cortical biopsy specimens obtained from Project by Lingeman; mRNA expression levels will be measured for VDR and CaR.