CORE--ASSAY

该核心测量血清、血清超滤液和尿液化学，并计算人（Coe和Lingeman的项目）和猪（Evan的项目）方案的尿液过饱和度（SS），以及进行血清草酸盐的测定（项目核心1）。核心还进行测定，以测量尿液中草酸钙和磷酸钙的亚稳性上限（Coe和Lingeman的项目）。核心测量血清和尿液中的碘酞酸盐，用于计算碘酞酸盐清除率（Coe项目）。将计算正常血糖-高胰岛素葡萄糖钳夹诱导的葡萄糖利用率（项目 由Coe）与糖尿病研究和培训中心合作。将在拟定GCRC方案（Coe项目）中研究的患者中测定外周血单核细胞维生素D受体水平（PBM VDR）。将对从Lingeman项目获得的肾皮质活检标本中的VDR、钙受体（CaR）、钙结合蛋白9和钙结合蛋白28进行蛋白质丰度测定（通过蛋白质印迹法）;将测量VDR和CaR的mRNA表达水平。

This core measures serum, serum ultrafiltrate, and urine chemistries and calculates urine supersaturations (SS) for human (Projects by Coe and Lingeman) and pig {Project by Evan) protocols, as well as performing assays of serum oxalate (Project core1). The core also performs assays to measure upper limit of metastability with respect to calcium oxalate and calcium phosphate in urine (Projects by Coe and Lingeman). The core measures iothalamate in serum and urine for calculation of iothalamate clearances (Project by Coe). Calculation of euglycemic-hyperinsulinemic glucose clamp induced glucose utilization will be done (Project by Coe) in collaboration with the Diabetes Research and Training Center. Peripheral blood monocyte vitamin D receptor levels (PBM VDR) will be determined in patients studied in the proposed GCRC protocols (Project by Coe). Assays of protein abundance (by Western blot)will be carried out for VDR, calcium receptor (CaR), calbindin 9 and calbindin 28 in renal cortical biopsy specimens obtained from Project by Lingeman; mRNA expression levels will be measured for VDR and CaR.