CORE--ANIMAL BREEDING AND SURGICAL FACILITY

显微外科核心(核心B)在实现本方案具体目标的战略中发挥核心作用。所有项目都依赖于核心B设施的技术专长，而这种技术专长是该计划的基础。由于这个项目最初是组织的，所有的研究人员都清楚地知道，为了在肾移植领域进行研究，每个人都必须培训一到两名移植技术人员，配备外科手术站，并独立维持动物种群。通过在一个项目项目中联合努力，该项目为饲养动物和进行移植手术提供了核心实验室，可以节省大量的资金和精力。这个 许多正在进行的合作，对移植领域研究的热情，以及开发核心设施的正当需要，为该计划项目的最初发展提供了动力。这些情况一直存在，事实上，对核心服务的热情和需求一直在稳步增加。 显微外科核心设施的主要作用仍然是向项目调查人员提供小鼠移植。在目前的提议中，核心将提供肾脏和心脏移植。该核心还提供了测量肾移植功能的能力，如个别提案中所规定的那样。移植技术和移植动物的实验利用在个别项目建议书中有详细描述。

The Microsurgical Core (Core B) plays a central role in the strategies for achieving the specific objectives of this Program. All projects depend on the technical expertise of the Core B facility and this technical expertise is the foundation for the Program. As this Program was originally organized, it became obvious to all the investigators that in order for them to pursue studies in the area of renal transplantation, each would have to train one or two transplant technicians, equip surgical stations, and maintain populations of animals independently. By combining efforts in a Program Project that provided a core laboratory for maintaining animals and performing transplant surgery, a tremendous amount of money and effort could be saved. The numerous ongoing collaborations, the enthusiasm for the pursuit of studies in the area of transplantation and the legitimate need for the development of a Core facility provided the impetus for the original development of this Program Project. These circumstances have persisted and in fact the enthusiasm and demand for the Core services have steadily increased. The primary role of the Microsurgical Core facility continues to be providing mouse transplants to Project investigators. For the current proposal, the core will provide both kidney and heart transplants. This Core also provides capabilities for measuring renal transplant function as specified within the individual proposals. The techniques for transplantation and the experimental utilization of transplanted animals is described in detail in the individual Project proposals.