develop insects as models study wound healing, infection and the wound microbiome.

开发昆虫作为研究伤口愈合、感染和伤口微生物组的模型。

• 批准号: 2592867
• 金额: --
• 依托单位: Brunel University London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2592867
• 关键词: develop; insects; models; study; wound

Burn wounds are extremely prevalent, almost everyone experiences a thermal injury during their lifetime. Burns are divided into degrees depending on the depth of the tissues that are being affected. Minor or first degree burns usually heal without any complications. However, once the thermal injury is sustained to deeper skin layers and with the increase of the burn surface area many challenges arise. Severe burns negatively affect most body systems, in particular the immune system, making the patient immune-compromised. This increases the chance of the burn wound becoming infected (Branski et al., 2009). Burn wound infection is the leading cause of mortality among burn survivors (Akers et al., 2019). In addition to that, it can lead to autograph failures as well as longer treatment courses. This puts a significant burden on the healthcare systems and the patient's wellbeing, which makes burn research a priority.The nature of burn wounds is complex due to a multi-system damage that it induces; therefore,it is impossible to recreate in vitro (Fransen et al., 2016). A number of in vivo models have been established to study the burn wound and infection. One of the most widely used models is murine. It is well-established and has already greatly improved our understanding of the burn physiology and pathology. The other well-studied model is the porcine model. Due to similarities in physiology and anatomy to humans, it has provided invaluable insights into burn injury and infection. The porcine model has also been used as ex vivo model (pig skin explants) to study the burn injury. Some studies have been conducted on canine and rabbit models as well. The abovementioned models have a multitude of advantages to them, ranging from body system similarities to versatility of the model organism. However, they have a few disadvantages in common such as: 1) they are strictly ethically regulated; 2) the size of the experimental cohort is very limited; 3) they are costly; among others. In addition to that, the burn wound injury is a severe and morbid condition which is very distressing to the animal(Abdullahi et al., 2014). This creates additional hurdles for researchers and therefore limits the burn wound research.

烧伤非常普遍，几乎每个人在一生中都会经历过热损伤。根据受影响组织的深度，烧伤分为不同程度。轻微或一度烧伤通常会愈合，不会出现任何并发症。然而，一旦热损伤持续到更深的皮肤层，并且随着烧伤表面积的增加，就会出现许多挑战。严重烧伤会对大多数身体系统产生负面影响，特别是免疫系统，使患者免疫系统受损。这增加了烧伤伤口感染的机会（Branski et al., 2009）。烧伤创面感染是烧伤幸存者死亡的主要原因（Akers 等，2019）。除此之外，它还可能导致签名失败以及更长的治疗疗程。这给医疗保健系统和患者的健康带来了沉重的负担，这使得烧伤研究成为当务之急。烧伤伤口的性质很复杂，因为它会引起多系统损伤；因此，不可能在体外重建（Fransen et al., 2016）。已经建立了许多体内模型来研究烧伤创面和感染。最广泛使用的模型之一是小鼠。它已经很成熟，并且已经极大地提高了我们对烧伤生理学和病理学的理解。另一个经过深入研究的模型是猪模型。由于生理学和解剖学与人类相似，它为烧伤和感染提供了宝贵的见解。猪模型也被用作离体模型（猪皮外植体）来研究烧伤。一些研究也在犬和兔模型上进行。上述模型具有许多优点，从身体系统的相似性到模型生物体的多功能性。然而，它们也有一些共同的缺点，例如：1）它们受到严格的道德监管； 2）实验队列的规模非常有限； 3）成本高昂；除其他外。除此之外，烧伤是一种严重且病态的情况，对动物来说非常痛苦（Abdullahi et al., 2014）。这给研究人员带来了额外的障碍，从而限制了烧伤创面的研究。