Core--Genomics/Proteomics Shared Resources

描述（由申请人提供）：基因组学/蛋白质组学共享资源核心联合董事：成员：Kenneth Beckman， CHORI Jim Kaput， UC - Davis博士Mark Shigenaga， CHORI A，概述基因组学/蛋白质组学/分析共享资源核心（以下简称“基因组学设施核心”）将为少数民族差异中心资助的成员集中和简化常规基因组学，蛋白质组学和小分子分析。提供的主要服务将集中于基因组学/蛋白质组学，包括SNP基因分型、通过微阵列和实时PCR分析基因表达，以及2-D/MALDI-TOF蛋白质表征。除了提供明确的研究服务外，核心工作人员还将监督中心任何成员进行非常规研究时使用核心设备的情况。集中这些工作的基本原理是利用CHORI最近对现有仪器的大量投资，并尽量减少成员实验室的重复工作。具体来说，核心预算将主要用于支助两名高技能研究助理的薪金，他们将使用现有设备提供服务。核心是两位联合董事

DESCRIPTION (provided by applicant): Genomics/Proteomics Shared Resources Core Co-Directors: Member: Kenneth Beckman, CHORI Jim Kaput, UC - Davis Dr. Mark Shigenaga, CHORI A, OVERVIEW The Genomics/Proteomics/Analytical Shared Resources Core (henceforth: the "genomics facility core") will centralize and streamline routine genomic, proteomic, and small molecule assays for members of the Minority Disparities Center Grant. Primary services offered will focus on genomics/proteomics and will include SNP genotyping, the analysis of gene expression by microarray and real-time PCR, and 2-D/MALDI-TOF protein characterization. In addition to providing a defined offering of research services, the core staff will supervise access to core equipment for non-routine research by any Center member. The rationale for centralizing such work is to leverage the large investments recently made in existing instrumentation at CHORI, and to minimize duplication of effort in member labs. Specifically, the core budget will primarily be used to support the salaries of two highlyskilled research associates, who will provide services using existing equipment. The core's two codirectors (Dr. Shigenaga and Dr. Beckman) will each supervise one technician, and will provide nonoverlapping functional expertise. Part of the budget will be used to provide on-going maintenance contracts for core instrumentation, to purchase consumables for use in validating and developing protocols for Center members, and to provide software and consumables for sample handling. Details of the objectives, rationale, facilities and equipment, capabilities, staffing, management and usage, and budget of the core are described below.