Core--Genomics/Proteomics Shared Resources

描述(由申请人提供)：基因组学/蛋白质组学共享资源核心联席董事：成员：Kenneth Beckman，CHORI Jim Kaput，UC-Davis Dr.Mark Shigaga，CHORI A，概述基因组学/蛋白质组学/分析共享资源核心(以下简称：“基因组设施核心”)将为少数族裔差异中心补助金成员集中和简化常规的基因组、蛋白质组和小分子分析。提供的主要服务将集中在基因组学/蛋白质组学方面，将包括SNP基因分型、通过微阵列和实时PCR分析基因表达以及2-D/MALDI-TOF蛋白质表征。除了提供特定的研究服务外，核心工作人员还将监督任何中心成员访问用于非常规研究的核心设备。集中这类工作的基本原理是利用最近在CHORI现有仪器上进行的大量投资，并将成员实验室的重复工作降至最低。具体而言，核心预算将主要用于支付两名高技能研究人员的工资，他们将利用现有设备提供服务。核心的两位联席董事 (Shigaga博士和Beckman博士)将每人指导一名技术人员，并将提供不重叠的功能专业知识。预算的一部分将用于为核心仪器提供持续的维护合同，购买消耗品，用于验证和制定中心成员的协议，并提供处理样品的软件和消耗品。下文详细说明了核心的目标、理由、设施和设备、能力、人员配置、管理和使用以及预算。

DESCRIPTION (provided by applicant): Genomics/Proteomics Shared Resources Core Co-Directors: Member: Kenneth Beckman, CHORI Jim Kaput, UC - Davis Dr. Mark Shigenaga, CHORI A, OVERVIEW The Genomics/Proteomics/Analytical Shared Resources Core (henceforth: the "genomics facility core") will centralize and streamline routine genomic, proteomic, and small molecule assays for members of the Minority Disparities Center Grant. Primary services offered will focus on genomics/proteomics and will include SNP genotyping, the analysis of gene expression by microarray and real-time PCR, and 2-D/MALDI-TOF protein characterization. In addition to providing a defined offering of research services, the core staff will supervise access to core equipment for non-routine research by any Center member. The rationale for centralizing such work is to leverage the large investments recently made in existing instrumentation at CHORI, and to minimize duplication of effort in member labs. Specifically, the core budget will primarily be used to support the salaries of two highlyskilled research associates, who will provide services using existing equipment. The core's two codirectors (Dr. Shigenaga and Dr. Beckman) will each supervise one technician, and will provide nonoverlapping functional expertise. Part of the budget will be used to provide on-going maintenance contracts for core instrumentation, to purchase consumables for use in validating and developing protocols for Center members, and to provide software and consumables for sample handling. Details of the objectives, rationale, facilities and equipment, capabilities, staffing, management and usage, and budget of the core are described below.