High Throughput Intracellular Drug Screening Platform

描述（由申请人提供）：本提案的主要目的是开发高通量细胞内药物筛选平台。Fluxion提出了一种快速、安全和有效的方法，通过穿过细胞膜进入完整细胞中的蛋白质，以促进作为潜在药物靶点的细胞内蛋白质的功能筛选。该提案的重点是开发一种微制造的单细胞电穿孔阵列，其将原本不可渗透的药物化合物引入细胞阵列中，以动态筛选化合物和蛋白质之间的相互作用。完成的阵列平台具有自动单细胞捕获和电穿孔、快速化合物插入以及多路实时光电监测等功能。该项目的具体目标是： I.确定有效的电穿孔条件，以打开细胞膜上的孔并使其重新密封。 二.确保药物化合物的最佳剂量。 三.改进用于多路检测和高通量的设备设计。

DESCRIPTION (provided by applicant): The main objective of this proposal is to develop a high-throughput intracellular drug screening platform. Fluxion proposes a rapid, safe, and efficient method to access proteins in the intact cell, by traversing the cell membrane, to facilitate functional screening of intracellular proteins as potential drug targets. This proposal focuses on developing a microfabricated single cell electroporation array which introduces otherwise impermeable drug compounds into the cellular array to dynamically screen for interactions between the compounds and the proteins. The completed array platform has functions including automatic single cell trapping and electroporation, rapid compound insertion, and multiplexed real-time optical and electrical monitoring. The specific aims of this project are to: I. Determine efficient electroporation conditions to open pores on the cell membrane and enable their resealing. II. Ensure optimal dosing of the drug compounds. III. Improve device design for multiplexing assays and for high-throughput.