NANO-WRINKLES AND PETALS ENHANCED FLUORESCENCE

纳米皱纹和花瓣增强荧光

• 批准号: 8171002
• 负责人: Michelle Khine
• 金额: $ 0.07万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171002
• 关键词: Biotin; Computer Retrieval of Information on Scientific Projects Database; Confocal Microscopy; Cultured Cells; Fluorescence; Funding; Glass; Gold; Grant; Image; Institution; Label; Metals; Photons; Research; Research Personnel; Resources; Source; Streptavidin; Time; United States National Institutes of Health; fluorescence imaging; nano; particle; two-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Immobilize streptavidin labeled Qdots on the gold wrinkles, petals and glass plates modified with biotin-BSA. Obtain fluorescence images and intensity time trace of single particle on each substrate by one-photon and two-photon confocal microscopies. Comparing the results to demonstrate metal nano-wrinkles and petals can enhance fluorescence emission. Culture cells with EGFP expression on the wrinkles and petals. Using wrinkles and petals as substrates for metal enhanced fluorescence cellar imaging.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 将链霉亲和素标记的量子点固定在用生物素-BSA修饰的金皱纹、花瓣和玻璃板上。 通过单光子和双光子共聚焦显微镜获得每个基底上单个颗粒的荧光图像和强度时间轨迹。 比较结果以证明金属纳米褶皱和 花瓣可以增强荧光发射。 在皱纹和花瓣上培养表达EGFP的细胞。 以皱纹和花瓣为基质进行金属增强荧光细胞成像。