System for Ocular Oxygen Measurements

眼氧测量系统

• 批准号: 6934880
• 负责人: Bruce M Ishimoto
• 金额: $ 12.19万
• 依托单位: OCUMETRICS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6934880
• 关键词: anterior chamber; automated data processing; biomedical equipment development; contact lens; diagnosis design /evaluation; eye; eye disorder diagnosis; fluorescent dye /probe; fluorimetry; laboratory rabbit; noninvasive diagnosis; oxygen tension; phosphorescence; photostimulus

DESCRIPTION (provided by applicant): The purpose of this project will be to develop a system for measuring oxygen concentrations on and inside the eye. This instrument will take advantage of phosphorescent probes that are sensitive to oxygen concentrations. This will have obvious applications in eye research and may eventually have applications in disease diagnosis. Our technique uses a dual frequency photon counting system, which we have developed specifically for this purpose. It combines maximum sensitivity with the ability to measure a wide range of oxygen concentrations. During Phase I, we will demonstrate feasibility of this technique both in vitro and in the rabbit eye. We will perform most of our work with a well established dye system, but will also begin to characterize additional dyes for further development during Phase II Our overall goal will be to develop multiple measurement systems based on dyes that have been selected for specific environmental affinity, oxygen range, etc. A critical goal will be standardization and calibration control, so that results made on different machines will be comparable. In addition, we will design this instrument with enough flexibility so that we can add capabilities as new phosphorescent oxygen probes are developed.

描述(申请人提供)：这个项目的目的是开发一种测量眼睛和眼睛内部氧气浓度的系统。该仪器将利用对氧浓度敏感的磷光探头。这将在眼科研究中有明显的应用，并最终可能在疾病诊断中应用。 我们的技术使用双频光子计数系统，这是我们专门为此目的开发的。它将最高灵敏度与测量大范围氧浓度的能力结合在一起。 在第一阶段，我们将在体外和兔眼中证明这项技术的可行性。 我们将使用成熟的染料系统进行大部分工作，但也将开始鉴定其他染料，以便在第二阶段进行进一步开发。 我们的总体目标将是开发基于特定环境亲和力、氧气范围等选择的染料的多种测量系统。关键目标将是标准化和校准控制，以便在不同机器上所做的结果具有可比性。此外，我们将以足够的灵活性设计这台仪器，以便我们可以在开发新的磷光氧探测器时增加功能。