Time Resolved Ocular Fluorometer

时间分辨眼荧光计

• 批准号: 6694019
• 负责人: Bruce M Ishimoto
• 金额: $ 10.26万
• 依托单位: OCUMETRICS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2004-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6694019
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; clinical research; diagnosis design /evaluation; eye disorder diagnosis; fluoresceins; fluorescent dye /probe; fluorimetry; human subject; lens; time resolved data

DESCRIPTION (provided by applicant): The purpose of this project will be to develop a general-purpose instrument for measuring fluorescence lifetimes on and inside the eye. This instrument will take advantage of fluorescence lifetime probes designed for microscopy and analytical chemistry to measure electrolytes, pH, glucose, oxygen and more in and on live eyes in animals and eventually humans. This will have obvious applications in eye research and may eventually have applications in disease diagnosis. During Phase I, we will demonstrate feasibility of this technique in the eye, as well as characterize the fluorescence lifetime of ocular autofluorescence. This characterization will be important to planning future projects since this autofluorescence generally will be "noise" to exogenous fluorescent sensors. Of course, in other research applications ocular autofluorescence could also be the target. Lens autofluorescence has been shown to be an indicator of diabetes, corneal autofluorescence of diabetic eye disease and fluorescent retinal lipofuscins have been implicated in the pathogenesis of Age Relate Macular Degeneration (AMD). Our overall goal will be to produce a standardized instrument and to develop protocols for using fluorescent lifetime sensors for making measurements in or on the eye. A critical goal will be standardization and calibration control, so that results made on different machines will be comparable. In addition, we will design this instrument with enough flexibility so that we can add capabilities as new fluorescence lifetime probes are developed, and our customers can develop techniques of their own.

描述（由申请人提供）：本项目的目的是开发一种通用仪器，用于测量眼睛上和眼睛内的荧光寿命。该仪器将利用专为显微镜和分析化学设计的荧光寿命探针来测量动物和最终人类活体眼睛中的电解质，pH值，葡萄糖，氧气等。这将在眼科研究中有明显的应用，并最终可能在疾病诊断中有应用。 在第一阶段，我们将证明这种技术在眼睛中的可行性，以及表征眼睛自发荧光的荧光寿命。这种表征对于规划未来的项目将是重要的，因为这种自发荧光通常对于外源荧光传感器是“噪声”。当然，在其他研究应用中，眼部自体荧光也可以是目标。透镜自发荧光已被证明是糖尿病的指标，糖尿病性眼病的角膜自发荧光和荧光视网膜脂褐素已被牵连在年龄相关性黄斑变性（AMD）的发病机制中。 我们的总体目标将是生产一个标准化的仪器，并开发协议，使用荧光寿命传感器进行测量的眼睛或眼睛。一个关键的目标将是标准化和校准控制，使不同的机器上的结果将是可比的。此外，我们将设计具有足够灵活性的仪器，以便我们可以在开发新的荧光寿命探针时增加功能，我们的客户可以开发自己的技术。