A Rapid On-Site Human Fecal Contamination Biosensor

描述(由申请人提供)： 人类粪便污染对伤寒、霍乱和甲型肝炎等水源性疾病的传播具有严重的健康危害。因此，对供水和食品加工过程中粪便微生物的敏感测量对于识别危险的人类粪便污染和追踪其来源具有重要价值。开发这样一种快速、便携的人类粪便污染生物传感器是该计划的总体目标。这项为期六个月的第一阶段计划的具体目标是：1)确定使用合成寡核苷酸的光纤消逝波DNA杂交分析的灵敏度、特异性和重用性；2)使用人和动物粪便样本确定针对类杆菌和普氏杆菌rRNA序列的光纤杂交分析的灵敏度和特异性；以及3)确定长期使用的检测成分的稳定性和重复性。

DESCRIPTION (provided by applicant): Human fecal contamination represents a serious health hazard for the transmission of waterborne diseases such as typhoid fever, cholera, and hepatitis A. Therefore, sensitive measurements of water supplies and food handling processes for fecal microorganism are of great value in identifying dangerous human fecal contamination and tracking its source. The development of such a rapid, portable, human fecal contamination biosensor is the overall goal of this program. The Specific Aims of this six-month Phase I program are: 1) Determine the sensitivity, specificity, and reuse of an optical fiber based evanescent wave DNA hybridization assay using synthetic oligos, 2) Determine the sensitivity and specificity of the optical fiber hybridization assay targeting Bacteroides and Prevotella rRNA sequences using human and animal fecal samples, and 3) Determine the stability and reproducibility of the assay components for long-term use.