A Rapid On-Site Human Fecal Contamination Biosensor

快速现场人类粪便污染生物传感器

• 批准号: 6879294
• 负责人: JACK R. U'REN
• 金额: $ 8.01万
• 依托单位: SAIGENE CORPORATION
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879294
• 关键词: Bacteroidaceae; Bacteroides; animal tissue; bacterial DNA; bacterial RNA; bioengineering /biomedical engineering; biohazard detection; biomedical equipment development; biosensor device; biotechnology; bioterrorism /chemical warfare; environmental contamination; feces analysis; fiber optics; food sanitation; human tissue; nucleic acid hybridization; oligonucleotides; portable biomedical equipment; water sampling /testing

DESCRIPTION (provided by applicant): Human fecal contamination represents a serious health hazard for the transmission of waterborne diseases such as typhoid fever, cholera, and hepatitis A. Therefore, sensitive measurements of water supplies and food handling processes for fecal microorganism are of great value in identifying dangerous human fecal contamination and tracking its source. The development of such a rapid, portable, human fecal contamination biosensor is the overall goal of this program. The Specific Aims of this six-month Phase I program are: 1) Determine the sensitivity, specificity, and reuse of an optical fiber based evanescent wave DNA hybridization assay using synthetic oligos, 2) Determine the sensitivity and specificity of the optical fiber hybridization assay targeting Bacteroides and Prevotella rRNA sequences using human and animal fecal samples, and 3) Determine the stability and reproducibility of the assay components for long-term use.

描述（由申请人提供）： 人类粪便污染是一种严重的健康危害，可传播伤寒、霍乱和甲型肝炎等水媒疾病。因此，对供水和食品加工过程中粪便微生物的灵敏检测对于识别危险的人类粪便污染并追踪其来源具有重要价值。这种快速，便携式，人类粪便污染的生物传感器的发展是这个计划的总体目标。这个为期六个月的第一阶段方案的具体目标是：1）使用合成的寡核苷酸测定基于光纤的渐逝波DNA杂交测定的灵敏度、特异性和重复使用，2）使用人和动物粪便样品测定靶向拟杆菌属和普雷沃氏菌属rRNA序列的光纤杂交测定的灵敏度和特异性，和3）确定长期使用的测定组分的稳定性和再现性。