DETERMINATION OF MUTATIONAL SPECTRA IN THE LACI GENE

LACI 基因突变谱的测定

• 批准号: 2852955
• 负责人: JACK R. U'REN
• 金额: $ 10万
• 依托单位: SAIGENE CORPORATION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-08 至 1999-08-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2852955
• 关键词: fluorescent dye /probe; gene expression; gene mutation; genetic screening; mutagens; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; oligonucleotides; restriction fragment length polymorphism; technology /technique development

DESCRIPTION (Adapted from investigator's abstract): The applicants propose development of an efficient hybridization screening test for mutations in the lacI gene based on a microwell transfer pin approach and a "probe" pooling strategy. The goal is to provide "mutational fingerprints or spectra" capable of defining levels and mechanism of mutation induced by individual mutagenic compounds. The test will use Stratagene's BigBlue transgenic system to recover mutants. However, the present proposal seeks to develop a strategy whereby fluorescein- labeled oligonucleotides probes complementary to selected lacI mutations are specifically annealed to biotin-labeled PCR products immobilized on streptavidin-coated pins. Positive hybridization events are revealed by a colorimetric reaction using anti-fluorescein-horse radish peroxidase conjugates in microwells. PROPOSED COMMERCIAL APPLICATION: Not available

描述（改编自研究者摘要）：申请人