Multi-frequency impedance sensor for cell analysis

用于细胞分析的多频阻抗传感器

• 批准号: 6933631
• 负责人: Jody V Vykoukal
• 金额: $ 9.79万
• 依托单位: ADEPTAS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933631
• 关键词: bioengineering /biomedical engineering; biomedical equipment; biomedical equipment development; blood cells; cell line; cell population study; dielectric property; electrical impedance; high throughput technology; human genetic material tag; human tissue; miniature biomedical equipment; portable biomedical equipment; tissue /cell culture

DESCRIPTION (provided by applicant): Broadband impedance analysis of individual cells yields a wealth of information about cell size, viability, morphology and interior structure. Cell dielectric properties including membrane conductivity, cytoplasmic conductivity, and membrane capacitance can be derived from multi-frequency impedance data and used to differentiate and enumerate cell populations in a mixed sample. Additionally, impedance analysis can be used to deduce cell differentiation, the onset of apoptosis, or cellular changes due to toxicant exposure. We have built a proof-of-principle particle analysis system that measures the impedance characteristics of individual cells at multiple frequencies as they pass through a sensor incorporated into a microfluidic flow channel. The goal of the effort described in this proposal is to improve our current particle impedance analyzer to yield a high-throughput, multipurpose unit for cell analysis. Specifically, a scalable impedance measurement circuit based on relatively low cost discrete components will be developed to allow for particle impedance spectroscopy at three frequencies. A more facile fabrication method for the impedance sensor channel will be developed to yield a credit-card sized, potentially disposable microfluidic device. The sensor channel cartridge and impedance sensing electronics will be integrated and the resulting prototype device used to characterize the dielectric properties of cultured cells and blood cell subpopulations. The long-term goal of this project is to realize an inexpensive, commercializable, hand-held multi-frequency particle impedance sensor that can be used for many cell analysis applications, including tissue culture, drug discovery, point-of-care diagnostics, and other scientific and industrial needs.

描述（由申请人提供）：单个细胞的宽带阻抗分析产生了大量关于细胞大小、活力、形态和内部结构的信息。包括膜电导率、细胞质电导率和膜电容的细胞介电性质可以从多频阻抗数据导出，并用于区分和计数混合样品中的细胞群体。此外，阻抗分析可用于推断细胞分化、细胞凋亡的发生或由于毒物暴露引起的细胞变化。我们已经建立了一个原理验证的颗粒分析系统，该系统在多个频率下测量单个细胞的阻抗特性，因为它们通过集成到微流体流动通道中的传感器。本提案中描述的努力的目标是改进我们当前的粒子阻抗分析仪，以产生用于细胞分析的高通量、多用途单元。具体地说，一个可扩展的阻抗测量电路的基础上，相对低成本的分立元件将被开发，以允许在三个频率的粒子阻抗谱。将开发一种更容易的阻抗传感器通道的制造方法，以产生信用卡大小的、可能是一次性的微流体装置。传感器通道盒和阻抗传感电子器件将被集成，并且所得到的原型装置用于表征培养细胞和血细胞亚群的介电特性。该项目的长期目标是实现一种廉价、可商业化的手持式多频粒子阻抗传感器，可用于许多细胞分析应用，包括组织培养、药物发现、即时诊断以及其他科学和工业需求。