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Rare Cell Isolation for Morphological & Molecular Study

稀有细胞的形态学分离

基本信息

批准号：
6783763
负责人：
Jody V Vykoukal
金额：
$ 13.71万
依托单位：
ADEPTAS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-08-01 至 2005-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6783763
关键词：
bioengineering /biomedical engineering biomedical equipment development biotechnology cell morphology cell population study cell sorting clinical research electrophoresis human tissue immunocytochemistry mass spectrometry matrix assisted laser desorption ionization microarray technology neoplastic cell stainings technology /technique development

项目摘要

DESCRIPTION (provided by applicant): A first step in the elucidation of the genetic changes and molecular profiles associated with the development of cancer is the isolation of rare tumor cells from a background of millions of normal cells, or the fractionation of a heterogeneous cell population into subpopulations. To this end we propose a versatile, dielectrophoresis-based cell fractionation and isolation platform that is an outgrowth of NCI IMAT funded studies currently underway at M.D. Anderson Cancer Center (MDACC). The goal of the project is to develop a device capable of separating human cells and presenting them in a familiar slide format appropriate for both cytomorphological analysis, which is still the gold standard for cancer diagnosis and staging, as well as a format that can serve as a front-end for emerging molecular analysis methods. The proposed electrosmear device utilizes a proprietary dielectrophoretic electrode array configuration and frequency scheme to levitate cells. This array is configured so that different cell types adhere to the slide in spatially distinct, characteristic bands, making the device ideal for isolating and grouping rare cells. Under an exclusive license from MDACC, Adeptas Inc. will design the appropriate electrodes and controlling electronics, design the sample chamber to permit an optional magnetophoretic separation component, assemble the device, and perform initial proof of concept tests with test particles. The biologically relevant testing of the device will be conducted at MDACC. Experiments at MDACC will utilize a variety of cultured tumor cell lines, whole blood, and buffy coat samples to demonstrate the ability of the electrosmear to isolate and trap cell subpopulations into characteristic bands based upon their dielectrophoretic properties. Key experiments will involve the isolation of tumor cells from spiked blood cell samples to test the limit of detection of the electrosmear method. The MDACC group will also test electrosmear slide compatibility with traditional cell staining methods, cytomorphological evaluation by both a pathologist and more recent automated slide counting cytometry methods, and molecular analysis methods including MALDI-TOF MS. Successful completion of this Phase I SBIR project should provide a basis for a Phase II application and commercialization of the device, which should be immediately applicable by pathologists and researchers in the life sciences.

描述（由申请人提供）：阐明与癌症发展相关的遗传变化和分子谱的第一步是从数百万正常细胞的背景中分离稀有肿瘤细胞，或将异质细胞群分成亚群。为此，我们提出了一种基于介电电泳的多功能细胞分级和分离平台，该平台是目前在 M.D. 安德森癌症中心 (MDACC) 正在进行的 NCI IMAT 资助研究的成果。该项目的目标是开发一种能够分离人类细胞并以熟悉的幻灯片格式呈现它们的设备，该格式适用于细胞形态学分析（这仍然是癌症诊断和分期的黄金标准），以及可以作为新兴分子分析方法前端的格式。所提出的电涂片装置利用专有的介电泳电极阵列配置和频率方案来悬浮细胞。该阵列的配置使得不同的细胞类型以空间上不同的特征带粘附在载玻片上，从而使该设备成为分离和分组稀有细胞的理想选择。根据 MDACC 的独家许可，Adeptas Inc. 将设计适当的电极和控制电子设备，设计样品室以允许使用可选的磁泳分离组件，组装设备，并使用测试粒子进行初步概念验证测试。该设备的生物学相关测试将在 MDACC 进行。 MDACC 的实验将利用各种培养的肿瘤细胞系、全血和血沉棕黄层样本来证明电涂片能够根据细胞亚群的介电泳特性将细胞亚群分离并捕获到特征条带中。关键实验将涉及从加标的血细胞样本中分离肿瘤细胞，以测试电涂片方法的检测限。 MDACC 小组还将测试电涂片载玻片与传统细胞染色方法的兼容性、病理学家的细胞形态学评估和更新的自动载玻片计数细胞术方法以及包括 MALDI-TOF MS 在内的分子分析方法。第一阶段 SBIR 项目的成功完成将为该设备的第二阶段应用和商业化奠定基础，该设备应立即被病理学家和生命科学领域的研究人员应用。