Nanoparticle System for High Throughput Proteomics

用于高通量蛋白质组学的纳米颗粒系统

• 批准号: 6881871
• 负责人: CYNTHIA C. BAMDAD
• 金额: $ 19.9万
• 依托单位: MINERVA BIOTECHNOLOGIES CORPORATION
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6881871
• 关键词: Nanoparticle; System; Throughput; Proteomics

DESCRIPTION (provided by applicant): Minerva Biotechnologies is a nanoparticle company that has developed proprietary technology that enables high throughput proteomics. Without the development of new enabling technologies, sequential testing of every possible pair-wise interaction would require about a billion experiments. However, assuming about 40,000 proteins in the proteome and each might interact with five real binding partners, we really only need to identify 160,000 interacting pairs. We reasoned that if we could stably attach a protein to its encoding DMA, and if we could isolate interacting partners from non-interacting proteins, the problem of generating a proteome-wide 3-dimensional interaction map would be soluble. The proposal under review combines the power of yeast 2-hybrid with the accuracy of in vitro testing. This proposal describes a complete nanotechnology system that will enable the mapping of large protein interaction networks in massively parallel format.

描述（由申请人提供）：Minerva Biotechnologies是一家纳米颗粒公司，已开发出可实现高通量蛋白质组学的专有技术。如果不开发新的启用技术，对每种可能的成对相互作用进行顺序测试将需要大约十亿个实验。但是，假设该蛋白质组中约有40,000个蛋白质，并且每个蛋白质可能与五个真正的结合伙伴相互作用，我们实际上只需要识别160,000对相互作用对即可。我们认为，如果我们可以稳定地将蛋白质连接到其编码DMA上，并且如果我们可以将相互作用的伴侣与非相互作用蛋白隔离开来，那么生成全蛋白质组的3维相互作用图的问题将是可溶的。审查中的提案结合了酵母2杂交的力量和体外测试的准确性。该提案描述了一个完整的纳米技术系统，该系统将以大量平行的格式绘制大型蛋白质相互作用网络。