Novel Isotopic Analyzer for Energy Expenditure Studies

用于能源消耗研究的新型同位素分析仪

• 批准号: 6885465
• 负责人: BRUCE ARAM RICHMAN
• 金额: $ 9.98万
• 依托单位: PICARRO, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6885465
• 关键词: bioenergetics; biomedical equipment development; hydrogen; measurement; oxygen; spectrometry

DESCRIPTION (provided by applicant): The doubly labeled water (DLW) method of measuring total energy expenditure (TEE) has emerged as a vital tool in obesity research. It has enabled researchers to assess human energy requirements in health and in disease, to study the etiology of obesity, and to validate tools for the assessment of dietary intake. Picarro proposes to develop novel instruments that will measure oxygen and hydrogen isotope ratios in water with high precision by cavity ringdown spectroscopy (CRDS). As compared with existing mass spectrometery techniques, the proposed instruments will not only lower the costs of DLW studies but also enable physicians and medical technicians to measure TEE of individual patients by the DLW method in clinical settings. In Phase I, Picarro will build an isotopic analyzer in order to identify the appropriate isotopic water absorption lines with high resolution, and demonstrate detection sensitivity of the H2O18 line in water with a signal to noise ratio of better than 1 part in 5000. In phase II, Picarro will develop compact prototypes suitable for deletion of both oxygen and hydrogen isotopes and compare their performance with conventional mass spectrometry in a clinical study. A fieldable instrument not requiring extensive sample preparation would greatly extend the applications reach of energy expenditure methodology. The instrument could also be used by manufacturers of heavy water for quality control of their product.

描述（由申请人提供）： 测量总能量消耗（TEE）的双标记水（DLW）方法已成为肥胖研究中的重要工具。它使研究人员能够评估人类健康和疾病的能量需求，研究肥胖的病因，并验证评估饮食摄入量的工具。Picarro建议开发新的仪器，通过腔衰荡光谱（CRDS）高精度地测量水中的氧和氢同位素比率。与现有的质谱技术相比，所提出的仪器不仅降低了DLW研究的成本，而且使医生和医疗技术人员能够在临床环境中通过DLW方法测量个体患者的TEE。在第一阶段，Picarro将建造一台同位素分析仪，以高分辨率识别适当的同位素吸水线，并证明水中H2O 18线的检测灵敏度，信噪比优于1/5000。在第二阶段，Picarro将开发适合删除氧和氢同位素的紧凑型原型，并在临床研究中将其性能与传统质谱法进行比较。不需要大量样品制备的现场仪器将大大扩展能量消耗方法的应用范围。该仪器也可用于重水制造商对其产品进行质量控制。