Novel Isotopic Analyzer for Energy Expenditure Studies

用于能源消耗研究的新型同位素分析仪

• 批准号: 7294889
• 负责人: BRUCE ARAM RICHMAN
• 金额: $ 25.51万
• 依托单位: PICARRO, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7294889
• 关键词: Adsorption; Adult; Alcohols; Amines; Ammonia; Anus; Arts; Austria; Carbon Dioxide; Child; Clinical; Clinical Research; Clinics and Hospitals; Compatible; Condition; Data Analyses; Deuterium; Diagnostic; Dietary intake; Disease; Energy Metabolism; Etiology; Exercise; Exhalation; Health; Human; Human Resources; Human body; Hydrogen; International Unit; Isotopes; Journals; Label; Laboratories; Left; Manufacturer Name; Mass Spectrum Analysis; Measurement; Measures; Medicine; Memory; Metabolic; Metabolism; Methodology; Methods; Monitor; Noise; Obesity; Optics; Outcome; Overweight; Oxygen; Oxygen Isotopes; Phase; Preparation; Production; Protocols documentation; Quality Control; Rate; Reporting; Research; Research Personnel; Sampling; Series; Spectrum Analysis; Standards of Weights and Measures; System; Techniques; Technology; Testing; Time; Training; Treatment Protocols; Universities; Urine; Vaporizer; Water; Wisconsin; Work; Writing; absorption; aqueous; college; cost; design; hydrology; improved; instrument; instrumentation; novel; nutrition; obesity treatment; programs; stable isotope; symposium; time interval; tool; vapor; volatile organic compound; water quality; water sampling

DESCRIPTION (provided by applicant): The doubly labeled water (DLW) method of measuring total energy expenditure (TEE) has emerged as a vital tool in obesity research. It has enabled researchers to assess human energy requirements in health and in disease, to study the etiology of obesity, and to validate tools for the assessment of dietary intake. Picarro proposes to develop novel instruments that will measure oxygen and hydrogen isotope ratios in water with high precision by cavity ring down spectroscopy (CRDS). Currently DLW analysis is performed by only 3 or 4 laboratories in the US, and DLW researchers must send their samples to one of these facilities for analysis. As compared with existing mass spectrometry techniques, the proposed instruments will reduce the cost and difficulty of DLW studies, thus enabling many researchers to perform DLW analysis in their own laboratories. They will be able to perform more research, more quickly, conveniently, and at lower cost. In addition, a field-ready instrument not requiring extensive sample preparation would greatly extend the application's reach of energy expenditure methodology. The instrument could also be used by manufacturers of isotopically enriched water for quality control of their product. The doubly labeled water (DLW) method of measuring total energy expenditure (TEE) has emerged as a vital tool in obesity research. The proposed instruments will reduce the time, cost, and difficulty of DLW studies, thus enabling many researchers to perform DLW analysis in their own laboratories. A field-ready instrument not requiring extensive sample preparation would greatly extend the application's reach of energy expenditure methodology.

描述（由申请人提供）：测量总能量消耗（TEE）的双标记水（DLW）方法已成为肥胖研究中的重要工具。它使研究人员能够评估人类健康和疾病的能量需求，研究肥胖的病因，并验证评估饮食摄入量的工具。Picarro建议开发新的仪器，通过腔衰荡光谱（CRDS）高精度地测量水中的氧和氢同位素比率。目前，DLW分析在美国只有3或4个实验室进行，DLW研究人员必须将他们的样品送到其中一个实验室进行分析。与现有的质谱技术相比，拟议的仪器将降低DLW研究的成本和难度，从而使许多研究人员能够在自己的实验室进行DLW分析。他们将能够进行更多的研究，更快，更方便，成本更低。此外，不需要大量样品制备的现场就绪仪器将大大扩展能量消耗方法的应用范围。该仪器也可用于同位素富集水的制造商，用于其产品的质量控制。测量总能量消耗（TEE）的双标记水（DLW）方法已成为肥胖研究中的重要工具。拟议的仪器将减少DLW研究的时间，成本和难度，从而使许多研究人员能够在自己的实验室进行DLW分析。不需要大量样品制备的现场就绪仪器将大大扩展能量消耗方法的应用范围。