The effect of membrane asymmetry on electron transfer in complex membrane proteins

膜不对称对复杂膜蛋白电子传递的影响

• 批准号: 2594980
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2594980
• 关键词: effect; membrane; asymmetry; electron; transfer

The asymmetric nature of biological membranes is emerging as an important factor in membrane protein function with the molecular basis and role of asymmetry remaining one of the most poorly understood phenomena in biology. We will investigate the impact of membrane asymmetry on the function and mechanisms of membrane-bound oxidoreductase enzymes at the heart of bioenergetics building on physical sciences innovation that will unlock a new microfluidic technology platform for reconstituting them into artificial membrane vesicles, with tunable lipid-composition, curvature and asymmetry. The development of novel pulse EPR approaches will allow the detection of low concentrations of spins from key radical intermediates. The technological innovations are expected to be broadly applicable to membrane proteins including redox- and metalloenzymes. The advances in understanding structural and mechanistic aspects of respiration and photosynthesis will pave the way to applications in medicine and energy technology.

生物膜的不对称特性是影响膜蛋白功能的重要因素，而不对称的分子基础和作用仍然是生物学中最不为人所知的现象之一。我们将研究膜不对称对生物能量学核心膜结合氧化还原酶的功能和机制的影响，建立在物理科学创新的基础上，这将打开一个新的微流控技术平台，将它们重新构建成具有可调脂质组成、曲率和不对称性的人工膜囊泡。新型脉冲EPR方法的发展将允许检测来自关键自由基中间体的低浓度自旋。这些技术创新有望广泛应用于膜蛋白，包括氧化还原酶和金属酶。在呼吸作用和光合作用的结构和机理方面的研究进展将为医学和能源技术的应用铺平道路。