TRANSCRIPTION FACTOR REGULATION BY THE BCR/ABL ONCOGENE

BCR/ABL 癌基因对转录因子的调节

• 批准号: 6952413
• 负责人: BRUNO CALABRETTA
• 金额: $ 31.44万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6952413
• 关键词: SCID mouse; biological signal transduction; cell differentiation; cell growth regulation; cell proliferation; chronic myelogenous leukemia; gene expression; gene mutation; hematopoietic stem cells; mutant; neoplastic cell; oncogenes; oncoproteins; polymerase chain reaction; proteasome; protein degradation; protooncogene; tissue /cell culture; transcription factor; ubiquitin

Transcription factor regulation by the BCR/ABL oncogene Human hematological malignancies are characterized by well- defined genetic abnormalities responsible for the generation of autonomous growth signals or the aberrant transduction of these signals from the cytoplasm to the nucleus. The BCR/ABL oncoproteins, the leukemia-specific gene products of the Philadelphia chromosome (Ph1) translocation, induce and maintain the leukemic phenotype through their deregulated tyrosine kinase activity; such activity is essential for recruitment and activation of multiple pathways that transduce signals leading to growth factor-independent proliferation, inhibition of apoptosis, and altered differentiation of myeloid precursor cells. The mechanisms of activation of the cytoplasmic downstream effectors of BCR/ABL are understood in some detail, but much less is known on the pathways leading to transcription factor regulation. This application focuses on investigating the BCR/ABL-dependent pathways leading to changes in the expression of c-Myb and C/EBPalpha, two transcription factors involved in the regulation of proliferation, survival, and differentiation of hematopoietic cells. Specifically, we will: 1) Investigate mechanisms of the enhanced expression/activity of c-Myb by: a. determining sequence requirements and enzymatic pathways that regulate ubiquitination and/or proteasome-dependent degradation of c-Myb in normal and BCR/ABL-expressing hematopoietic cells. b. identifying the interacting proteins promoting the ubiquitin/proteasome-dependent degradation of c-Myb. c. determining whether the activity of proteins promoting c-Myb degradation is modulated in BCR/ABL-expressing cells. 2) Assessing the effects of degradation-resistant c-Myb mutants on proliferation, survival, and differentiation of hematopoietic cells. 3) Assessing leukemic samples for the presence of c-myb mutations within domains involved in protein degradation. 4) Investigating the mechanisms whereby BCR/ABL suppresses the expression of the granulocytic differentiation regulator C/EBPalpha.

BCR/ABL癌基因对转录因子的调控人类血液恶性肿瘤的特点是具有明确的遗传异常，这些遗传异常负责产生自主生长信号或这些信号从细胞质到细胞核的异常转导。BCR/ABL癌蛋白是费城染色体（Ph1）易位的白血病特异性基因产物，通过解除酪氨酸激酶活性诱导和维持白血病表型；这种活性对于募集和激活多种途径至关重要，这些途径可转导导致生长因子不依赖的增殖、抑制凋亡和改变髓前体细胞分化的信号。BCR/ABL细胞质下游效应物的激活机制已经有了一些详细的了解，但对导致转录因子调节的途径知之甚少。本应用的重点是研究BCR/ abl依赖性通路导致C - myb和C/EBPalpha表达变化，这两种转录因子参与造血细胞增殖、存活和分化的调节。具体来说，我们将：1)研究c-Myb表达/活性增强的机制：a.确定序列要求和调节正常和表达BCR/ abl的造血细胞中c-Myb泛素化和/或蛋白酶体依赖性降解的酶促途径。b.鉴定促进泛素/蛋白酶体依赖性c-Myb降解的相互作用蛋白。c.确定促进c-Myb降解的蛋白活性是否在表达BCR/ abl的细胞中被调节。2)评估抗降解c-Myb突变体对造血细胞增殖、存活和分化的影响。3)评估白血病样本中c-myb突变在蛋白质降解区域的存在。4)探讨BCR/ABL抑制粒细胞分化调节因子C/EBPalpha表达的机制。