Functional Mapping of Ethanol Avoidance in Mouse Pain

描述（由申请方提供）：当比较两种小鼠品系时，观察到相反的乙醇自我给药行为：C57 BL/6 J x NZB/B1 NJ F1杂交小鼠（B6 xNZB）在经历高浓度乙醇后避免乙醇（回避者），而C57 BL/6 J x FVB/NJ F1杂交小鼠（BGxFVB）则没有（狂欢者）。本申请的首要假设是，这些杂交体在对乙醇的厌恶性而非奖励性或抗焦虑性的敏感性方面不同，并且特定细胞类型和脑区域中FosB和deltaFosB的差异水平反映了自愿乙醇自我施用中的经验依赖性差异。将通过采用以下测试来确定对乙醇的厌恶、奖励和抗焦虑特性的杂交敏感性;条件性味觉厌恶、条件性位置偏好、改良的两瓶选择和高架十字迷宫。为了定义由乙醇回避和狂欢参与的神经回路，将在乙醇经历的杂种中测量FosB和deltaFosB免疫反应性。还将确定FosB和deltaFosB表达神经元的神经元细胞类型。将确定FosB/deltaFosB阳性细胞核的应变、剂量和数量（每个脑区域和细胞类型）的影响。这些实验将有助于了解酒精的使用和滥用。

DESCRIPTION (provided by applicant): Opposing ethanol self-administration behaviors are observed when comparing two strains of mice: C57BL/6J x NZB/B1NJ F1 hybrids (B6xNZB) avoid ethanol (avoiders) after experience with high concentrations of ethanol and C57BL/6J x FVB/NJ F1 hybrids (BGxFVB) do not (revelers). The overarching hypothesis of this application is that these hybrids differ in sensitivity to the aversive, but not rewarding or anxiolytic, properties of ethanol and differential levels of FosB and deltaFosB in specific cell types and brain regions reflects experience dependent differences in voluntary ethanol self-administration. Hybrid sensitivity to aversive, rewarding, and anxiolytic properties of ethanol will be determined by employing the following tests; conditioned taste aversion, conditioned place preference, modified two bottle choice, and elevated plus maze. To define neurocircuits engaged by ethanol avoidance and revelry, FosB and deltaFosB immunoreactivity will be measured in ethanol experienced hybrids. Neuronal cell types of FosB and deltaFosB expressing neurons will also be determined. Effects of strain, dose, and quantity of FosB/deltaFosB positive nuclei (per brain region and cell type) will be determined. These experiments will contribute to the understanding of alcohol use and abuse.