Functional Mapping of Ethanol Avoidance in Mouse Pain

描述（由申请人提供）：在比较两种小鼠时观察到相反的乙醇自我给药行为：C57BL/6J xNZB /B1NJ F1杂交种（B6xNZB）在经历高浓度乙醇后避免乙醇（回避者），而C57BL/6J xFVB /NJ F1杂交种（BGxFVB）不（狂欢者）。该应用的总体假设是，这些杂交体对乙醇的厌恶性敏感性不同，但不具有奖励性或抗焦虑性，特定细胞类型和大脑区域中FosB和deltaFosB水平的差异反映了自愿乙醇自我给药的经验依赖差异。对乙醇的厌恶，奖励和焦虑特性的杂交敏感性将通过采用以下测试来确定；条件性味觉厌恶、条件性地点偏好、改良的两瓶选择和升高的加迷宫。为了确定乙醇回避和狂欢所参与的神经回路，将在乙醇经历的杂交种中测量FosB和deltaFosB的免疫反应性。还将确定表达FosB和deltaFosB神经元的神经元细胞类型。将确定菌株、剂量和FosB/deltaFosB阳性细胞核数量（每个脑区和细胞类型）的影响。这些实验将有助于了解酒精的使用和滥用。

DESCRIPTION (provided by applicant): Opposing ethanol self-administration behaviors are observed when comparing two strains of mice: C57BL/6J x NZB/B1NJ F1 hybrids (B6xNZB) avoid ethanol (avoiders) after experience with high concentrations of ethanol and C57BL/6J x FVB/NJ F1 hybrids (BGxFVB) do not (revelers). The overarching hypothesis of this application is that these hybrids differ in sensitivity to the aversive, but not rewarding or anxiolytic, properties of ethanol and differential levels of FosB and deltaFosB in specific cell types and brain regions reflects experience dependent differences in voluntary ethanol self-administration. Hybrid sensitivity to aversive, rewarding, and anxiolytic properties of ethanol will be determined by employing the following tests; conditioned taste aversion, conditioned place preference, modified two bottle choice, and elevated plus maze. To define neurocircuits engaged by ethanol avoidance and revelry, FosB and deltaFosB immunoreactivity will be measured in ethanol experienced hybrids. Neuronal cell types of FosB and deltaFosB expressing neurons will also be determined. Effects of strain, dose, and quantity of FosB/deltaFosB positive nuclei (per brain region and cell type) will be determined. These experiments will contribute to the understanding of alcohol use and abuse.