Intrinsic properties of olfactory bulb mitral cells

嗅球二尖瓣细胞的内在特性

• 批准号: 7068514
• 负责人: Ramani Balu
• 金额: $ 2.66万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7068514
• 关键词: action potentials; biological signal transduction; computer data analysis; computer program /software; electrophysiology; electrostimulus; laboratory rat; membrane channels; neural information processing; neurons; neurophysiology; olfactory lobe; potassium channel; sensory mechanism; statistics /biometry; synapses; voltage /patch clamp

DESCRIPTION (provided by candidate): The mammalian olfactory bulb is an ideal system to study general principles of sensory coding. Olfactory bulb circuits convert simple monotonic sensory input from olfactory receptor neurons into complex spatiotemporal patterns of mitral cell activation that are relayed to higher order brain centers. Very little is known about how the olfactory bulb produces this transformation. This application seeks to investigate the intrinsic electrophysiological properties of mitral cells-the principal output neurons of the olfactory bulb-and analyze how these properties pattern mitral cell responses. Using electrophysiological approaches, we will address two key questions: 1) what are the kinetic properties and molecular identity of slowly inactivating K+currents in mitral cells; and 2) how do recurrent and lateral synaptic inhibition interact with intrinsic currents in mitral cells to modulate spike timing and action potential propagation? We expect that the results from these experiments will shed important light on how the olfactory bulb processes sensory information, unveil general principles of how local circuits in the brain interact to perform computational tasks, and aid in the development of paradigms for therapeutic electrical stimulation to treat neurological diseases.

描述（由候选人提供）：哺乳动物嗅球是研究感觉编码一般原理的理想系统。嗅球回路将来自嗅觉受体神经元的简单单调感觉输入转化为复杂的二尖瓣细胞激活的时空模式，并将其传递给高级脑中心。关于嗅球是如何产生这种转变的，我们知之甚少。本应用程序旨在研究二尖瓣细胞（嗅球的主要输出神经元）的内在电生理特性，并分析这些特性如何影响二尖瓣细胞的反应。利用电生理学方法，我们将解决两个关键问题：1)二尖瓣细胞中K+电流缓慢失活的动力学特性和分子特性是什么；2)复发性和侧性突触抑制如何与二尖瓣细胞内禀电流相互作用以调节尖峰时间和动作电位的传播？我们期望这些实验的结果将对嗅球如何处理感觉信息，揭示大脑局部电路如何相互作用以执行计算任务的一般原理，并有助于开发治疗性电刺激治疗神经系统疾病的范例。