The Action of Chinese Medicinal Herbs on Prostate Cancer

说明(申请人提供)：中草药已在世界上得到广泛应用。然而，这些中草药的疗效和作用机制还没有得到很好的研究。必须使用标准化设计来研究它们的疗效，并利用现代技术来阐明它们的作用机制。通过使用抗肿瘤中药配方ZYD88，本研究方案旨在验证ZYD88诱导前列腺癌LNCaP肿瘤移植动物模型中前列腺癌细胞凋亡的假说。细胞凋亡将通过检测caspase-3激活和CK18(常见的早期凋亡事件)和DNA片段化(肿瘤组织切片中细胞凋亡的标志)来确定。此外，还将通过Western blotting分析细胞色素c从线粒体向胞浆的释放，以及细胞中Bcl2家族蛋白(Bcl2、Bc1-xl、Bax和Bak)的水平，以及细胞凋亡途径中的事件。将ZYD88处理的样品和未处理的样品进行数据比较。将进行ZYD88作用的剂量-反应和时间-过程分析。这些研究将有助于我们了解中草药在癌症治疗中的疗效和机制，这是我的职业生涯目标。

DESCRIPTION (provided by applicant): Chinese herbal medicines have been widely used in the world. However, the efficacy and mechanisms of these herbal medicines are not well studied. It is imperative to investigate their efficacy using standardized design, and to elucidate the mechanisms of their action using modern technology. By employing an anti-tumor Chinese herbal formula, ZYD88, the current research proposal is designed to test the hypothesis that ZYD88 induces apoptosis in prostate cancer cells in a xenograft animal model with prostate LNCaP tumor. Cell apoptosis will be determined by assaying caspase-3 activation and CK18, the common early apoptotic events, and DNA fragmentation, a hallmark of cell apoptosis in tumor tissue sections. Furthermore, the release of cytochrome c from mitochondria to cytosol and the cellular levels of Bcl-2 family proteins (Bcl-2, BcI-XL, Bax and Bak), events in apoptotic pathway will be determined by Western blotting analysis. The data will be compared between ZYD88 treated and untreated samples. Dose-response and time-course analyses of ZYD88 action will be performed. These studies will facilitate our understanding of the efficacy and mechanisms of Chinese herbal medicine in cancer therapy, a goal of my career.