The Action of Chinese Medicinal Herbs on Prostate Cancer

中药对前列腺癌的作用

• 批准号: 6834758
• 负责人: YAN-WEN DUAN
• 金额: $ 6.1万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-16 至 2007-03-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6834758
• 关键词: China; alternative medicine; antineoplastics; apoptosis; athymic mouse; combination chemotherapy; dosage; drug screening /evaluation; folk medicine; medicinal plants; neoplasm /cancer chemotherapy; neoplastic growth; nonhuman therapy evaluation; pharmacokinetics; postdoctoral investigator; prostate neoplasms; western blottings

DESCRIPTION (provided by applicant): Chinese herbal medicines have been widely used in the world. However, the efficacy and mechanisms of these herbal medicines are not well studied. It is imperative to investigate their efficacy using standardized design, and to elucidate the mechanisms of their action using modern technology. By employing an anti-tumor Chinese herbal formula, ZYD88, the current research proposal is designed to test the hypothesis that ZYD88 induces apoptosis in prostate cancer cells in a xenograft animal model with prostate LNCaP tumor. Cell apoptosis will be determined by assaying caspase-3 activation and CK18, the common early apoptotic events, and DNA fragmentation, a hallmark of cell apoptosis in tumor tissue sections. Furthermore, the release of cytochrome c from mitochondria to cytosol and the cellular levels of Bcl-2 family proteins (Bcl-2, BcI-XL, Bax and Bak), events in apoptotic pathway will be determined by Western blotting analysis. The data will be compared between ZYD88 treated and untreated samples. Dose-response and time-course analyses of ZYD88 action will be performed. These studies will facilitate our understanding of the efficacy and mechanisms of Chinese herbal medicine in cancer therapy, a goal of my career.

性状（申请人提供）：中草药在世界范围内应用广泛。然而，这些草药的功效和机制还没有得到很好的研究。必须使用标准化设计研究其功效，并使用现代技术阐明其作用机制。本研究采用抗肿瘤中药复方ZYD 88，在前列腺LNCaP肿瘤异种移植动物模型中验证ZYD 88诱导前列腺癌细胞凋亡的假设。细胞凋亡将通过测定胱天蛋白酶-3活化和CK 18（常见的早期凋亡事件）以及DNA片段化（肿瘤组织切片中细胞凋亡的标志）来确定。此外，细胞色素c从线粒体释放到胞质溶胶以及Bcl-2家族蛋白（Bcl-2、BcI-XL、Bax和巴克）的细胞水平、细胞凋亡途径中的事件将通过蛋白质印迹分析来确定。将比较ZYD 88处理和未处理样品之间的数据。将进行ZYD 88作用的剂量反应和时间过程分析。这些研究将有助于我们了解中草药在癌症治疗中的疗效和机制，这是我职业生涯的目标。