TISSUE PROCUREMENT CORE FACILITY AT JOSEPHINE FORD CANCE

约瑟芬福特坎斯的组织采购核心设施

• 批准号: 6873022
• 负责人: RICHARD ZARBO
• 金额: $ 26.27万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6873022
• 关键词: biomedical facility; cancer information system; cancer registry /resource; human tissue; tissue /organ preservation; tissue resource /registry

DESCRIPTION: (Applicant's Description) The exceptionally high benefit to cost ratio of a centrally operated tissue procurement core facility is well recognized and the need for a core facility at Henry Ford Health System (HFHS) has been acknowledged for some time. On January 15/1999, the Core Facility, a service of the Josephine Ford Cancer Center (JFCC) and housed within the Department of Pathology, became operational. A governing board of 5 members oversees activities of the Facility. We have focused initially on procuring tissue from surgeries performed only at the main hospital to assure the high quality of tissue management. A l2cc blood sample is collected at the time of pre-operative diagnostic blood work. On the day of surgery, an on-call pathologist's assistant collects tissue from operating rooms immediately after completion of the surgery. Each specimen is dissected according to surgical pathology protocol under direct supervision of a pathologist. Normal and malignant sections are separated; tumor samples are further evaluated for necrosis and/or hemorrhage. A separate paraffin block cassette/sample is prepared; blood and tissue specimens are aliquoted into multiple vials and snap frozen at - 80 C. Clinical and demographic data, number of vials/case, reason(s) for not allocating tissue for banking, procurement time, and the availability of matched blood samples are entered into appropriate databases. In the first 6 months, we collected a total of 347 specimens, 39% from African-American and 55% from Caucasian patients. The distribution of tissue by sites includes 17% prostate, 14% colon, 11% breast, 14% lung, and 38% from other organs. Tumor tissue viability ranged between 90- 100% with diagnostic needs as the main reason for not banking. The mean procurement time was 21.5 minutes (+ 3.19). In this application, we propose to continue and expand the operation the Core Facility with 3 foci. First, to strengthen the existing support system for researchers at JFCC. Second, to bank frozen tumor tissues with matched blood and paraffin embedded samples on a routine basis; and third, to accurately interface pathology, treatment outcome and epidemiologic data while maintaining patients' confidentiality.

描述：（申请人的描述）成本效益极高 中央操作的组织采购核心设施的比率良好 认识到亨利福特卫生系统（HFHS）需要一个核心设施 已经有一段时间了。1999年1月15日，核心基金a 约瑟芬福特癌症中心（JFCC）的服务，并设在 病理学系开始运作。由5名成员组成的管理委员会 监督设施的活动。 我们最初的重点是从手术中获取组织， 确保组织管理的高质量。A l2cc 在术前诊断血液工作时采集血样。 在手术当天，一名待命的病理学家助理从 手术完成后立即进入手术室。每个试样 在以下人员的直接监督下，根据外科病理学方案进行解剖 病理学家将正常和恶性切片分开;将肿瘤样品分离。 进一步评估坏死和/或出血。单独的石蜡块 准备好试剂盒/样本;将血液和组织样本分装到 多个小瓶并在-80 ℃快速冷冻。临床和人口统计学数据， 小瓶数量/病例，未分配组织用于库存的原因， 输入采购时间和匹配血液样本的可用性 到适当的数据库。在前6个月，我们共收集了347份 39%来自非洲裔美国人，55%来自白人患者。的 组织的部位分布包括17%的前列腺，14%的结肠，11%的乳腺， 14%来自肺，38%来自其他器官。肿瘤组织存活率范围为90- 100%， 100%，诊断需求是不存钱的主要原因。平均 采购时间为21.5分钟（+ 3.19）。 在本申请中，我们建议继续和扩大核心业务， 设施有3个焦点。第一，加强现有的支援系统， JFCC的研究人员。第二，用匹配的血液储存冷冻的肿瘤组织 和石蜡包埋样本;第三， 接口病理学、治疗结果和流行病学数据， 为病人保密