Traffic of catecholamine storage vesicle proteins
儿茶酚胺储存囊泡蛋白的运输
基本信息
- 批准号:6858705
- 负责人:
- 金额:$ 12.18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-06-01 至 2007-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant):
The chromogranin/secretogranins (or "granins") are a family of regulated secretory proteins ubiquitously found in the cores of amine and peptide hormone and neurotransmitter secretory vesicles. Evidence has now been gathered in support of both intracellular and extracellular functions for this protein family. Within the cells of origin, a granulogenic or sorting role in the regulated pathway of hormone or neurotransmitter secretion has been documented, especially for its index member, chromogranin A (CgA). Granins also function as pro-hormones, giving rise by proteolytic processing to a large array of peptide fragments for which diverse autocrine, paracrine, and endocrine activities have been demonstrated in vitro, ex vivo and more recently in vivo. For example, CgA fragments vasostatin and catestatin control vasoreactivity and catecholamine release, and the CgA fragment pancreastatin elevates blood glucose. This proposal develops 2 specific aims, employing novel granins chimeric proteins, to investigate in situ, mechanisms whereby chromogranin (CgA) is sorted into catecholamine storage granule of sympathoadrenal chromaffin cells, and interacts with other secreted or membrane proteins of the secretory granule. The responses to be investigated are exocytosis and FRET (fluorescence resonance energy transfer) molecular communication resulting from protein-protein interaction. In aim 1, we will use a series of CgA domains tagged with green fluorescent protein (GFP) or embryonic alkaline phosphatase (EAP), to identify in situ, the putative sorting signals within the primary structure of CgA (cis determinant) that mediate CgA's targeting to the chromaffin granule. In aim 2, granin chimeric proteins will be used to investigate in the living cell, which determinants of the secretory apparatus (trans determinants) interact with CgA to influence sorting of the granin into the regulated secretory pathway and its storage within the core of the catecholamine storage granule. The results of these studies will enhance our knowledge of catecholamine/granins storage and release during sympathetic stimulation, leading to a better understanding of neuroendocrine secretory granule biogenesis .
描述(由申请人提供):
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Role of H+-ATPase-mediated acidification in sorting and release of the regulated secretory protein chromogranin A: evidence for a vesiculogenic function.
H-ATP酶介导的酸化在受调节的分泌蛋白嗜铬粒蛋白A的分类和释放中的作用:产生囊泡功能的证据。
- DOI:10.1074/jbc.m408197200
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Taupenot,Laurent;Harper,KimberlyL;O'Connor,DanielT
- 通讯作者:O'Connor,DanielT
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LAURENT TAUPENOT其他文献
LAURENT TAUPENOT的其他文献
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{{ truncateString('LAURENT TAUPENOT', 18)}}的其他基金
Trafficking of catecholamine storage vesicle proteins
儿茶酚胺储存囊泡蛋白的贩运
- 批准号:
7844957 - 财政年份:2009
- 资助金额:
$ 12.18万 - 项目类别:
CELLULAR ROLE OF GRANINS IN HORMONE AND NEUROTRANSMITTER STORAGE AND R
谷粒蛋白在激素和神经递质储存和 R 中的细胞作用
- 批准号:
7957615 - 财政年份:2009
- 资助金额:
$ 12.18万 - 项目类别:
CELLULAR ROLE OF GRANINS IN HORMONE AND NEUROTRANSMITTER STORAGE AND R
谷粒蛋白在激素和神经递质储存和 R 中的细胞作用
- 批准号:
7722437 - 财政年份:2008
- 资助金额:
$ 12.18万 - 项目类别:
CELLULAR ROLE OF GRANINS IN HORMONE AND NEUROTRANSMITTER STORAGE AND R
谷粒蛋白在激素和神经递质储存和 R 中的细胞作用
- 批准号:
7601093 - 财政年份:2007
- 资助金额:
$ 12.18万 - 项目类别:
Trafficking of catecholamine storage vesicle proteins
儿茶酚胺储存囊泡蛋白的贩运
- 批准号:
7122644 - 财政年份:2005
- 资助金额:
$ 12.18万 - 项目类别:
Traffic of catecholamine storage vesicle proteins
儿茶酚胺储存囊泡蛋白的运输
- 批准号:
6772916 - 财政年份:2004
- 资助金额:
$ 12.18万 - 项目类别:
STIMULUS-EVENT COUPLING IN CHROMAFFIN SECRETORY GRANULES
嗜铬分泌颗粒中的刺激事件耦合
- 批准号:
6517915 - 财政年份:2001
- 资助金额:
$ 12.18万 - 项目类别:
STIMULUS-EVENT COUPLING IN CHROMAFFIN SECRETORY GRANULES
嗜铬分泌颗粒中的刺激事件耦合
- 批准号:
6635378 - 财政年份:2001
- 资助金额:
$ 12.18万 - 项目类别:
STIMULUS-EVENT COUPLING IN CHROMAFFIN SECRETORY GRANULES
嗜铬分泌颗粒中的刺激事件耦合
- 批准号:
6322497 - 财政年份:2001
- 资助金额:
$ 12.18万 - 项目类别:
Trafficking of catecholamine storage vesicle proteins
儿茶酚胺储存囊泡蛋白的贩运
- 批准号:
7430286 - 财政年份:
- 资助金额:
$ 12.18万 - 项目类别:
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