Structural studies of the RAD6/RAD18 complex

RAD6/RAD18 复合物的结构研究

• 批准号: 6791399
• 负责人: SACHA N ULJON
• 金额: $ 4.13万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6791399
• 关键词: DNA binding protein; DNA damage; DNA repair; X ray crystallography; biophysics; cancer prevention; nuclear magnetic resonance spectroscopy; protein binding; protein purification; protein structure; pyrimidine dimers; ultraviolet radiation

DESCRIPTION (provided by applicant): Ultraviolet light damages cellular DNA, primarily by forming dimers between pyrimidine base pairs. UV-induced DNA repair mechanisms are responsible for correcting this DNA damage and preventing cancer formation. The RAD6/RAD18 complex is the central director of these UV-induced DNA repair mechanisms. RAD18 binds damaged DNA, RAD6 binds RAD18, and the complex induces both error-free and error-prone mechanisms of DNA repair. My aim is to determine the structure of the RAD6/RAD18 heterodimeric complex using X-ray crystallographic methods.

描述(申请人提供)：紫外线损伤细胞DNA，主要是通过在嘧啶碱基对之间形成二聚体。紫外线诱导的DNA修复机制负责纠正这种DNA损伤并防止癌症形成。RAD6/RAD18复合体是这些紫外线诱导的DNA修复机制的中心指挥。RAD18结合受损的DNA，RAD6结合RAD18，该复合体诱导DNA修复的无错误和易错误机制。我的目标是用X射线结晶学方法确定RAD6/RAD18异二聚体的结构。