DNA-based information storage based on surface immobilised DNA

基于表面固定化DNA的DNA信息存储

• 批准号: 2599290
• 金额: --
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2599290
• 关键词: DNA; based; information; storage; surface

The exponential increase in digital data requires fundamentally new technology for long-term data archiving. While the concept of using DNA for non-volatile storage was introduced almost 25 years ago, only recently have advances in DNA synthesis and sequencing made such nucleic acid memories (NAMs) a viable proposition. However, at present, no end-to-end solution that can be integrated into existing silicon, IT technology exists. This project will investigate an end-to-end, hybrid semiconductor-NAM where information can be stored and processed in both the electronic and molecular domains and can move freely across the interface between domains. This project will focus on two challenges that underpin this vision: 1) design and optimisation of a DNA memory based on a library of DNA hairpins; 2) experimental demonstration of electronic query of the hairpin NAM through the use of pH-dependent DNA switches to electronically actuate key-based searching. This will be a highly experimental project using a range of techniques common to DNA nanotechnology including fluorescence and circular dichroism to explore DNA hairpin opening and quartz crystal microbalance with dissipation monitoring and TIRF microscopy to explore triplex switching as a function of pH. This programme is interdisciplinary with the potential for impact across multiple EPSRC themes, including Information and communication technologies, Microelectronics device technology and Biophysics and soft matter physics. Additionally, hybrid electronic-DNA technologies have particular relevance in medical diagnostics e.g., DNA aptamer-based biosensors. This project thus also contributes indirectly to the EPSRC Healthcare Technologies theme and the EPSRC Grand Challenge Understanding the Physics of Life.

数字数据的指数级增长需要全新的技术来实现长期数据存档。虽然使用DNA进行非易失性存储的概念在近25年前就被引入，但直到最近，DNA合成和测序的进展才使这种核酸存储器（NAM）成为可行的提议。然而，目前还没有可以集成到现有硅IT技术中的端到端解决方案。该项目将研究一种端到端的混合半导体-NAM，其中信息可以在电子和分子域中存储和处理，并且可以在域之间的界面上自由移动。该项目将重点关注支撑这一愿景的两个挑战：1）基于DNA发夹库的DNA存储器的设计和优化; 2）通过使用pH依赖性DNA开关以电子方式启动基于密钥的搜索，对发夹NAM的电子查询进行实验演示。这将是一个高度实验性的项目，使用一系列DNA纳米技术常见的技术，包括荧光和圆二色性，以探索DNA发夹开口和石英晶体微天平与耗散监测和TIRF显微镜，以探索三重开关作为pH值的函数。微电子器件技术和生物物理学和软物质物理学。此外，混合电子DNA技术在医学诊断中具有特别的相关性，DNA适体生物传感器因此，该项目也间接有助于EPSRC医疗保健技术主题和EPSRC理解生命物理学的大挑战。