Core--Confocal Imaging- Shared Resource

共聚焦核心的目的是加强Karmanos癌症研究所成员的同行评审资助的研究活动，其研究需要共聚焦显微镜和相关技术。能力和服务包括：共聚焦显微镜、高分辨率常规光学显微镜、体内小动物成像、原位杂交、细胞化学、组织化学、免疫细胞化学、免疫组织化学、细胞内运输研究、多重标记研究（最多3项，即将扩展至7项，可同时研究不同参数）和比率分析（例如，细胞内pH和离子测量研究），以及三维图像重建和定量测量。共聚焦成像系统由配备有三个激光器的Zeiss LSM 310共聚焦显微镜组成， 一台配备一台激光器的Meridian InsightPlus共聚焦显微镜和新收购的Zeiss LSM 510 Meta共聚焦显微镜，后者配备4台激光器和多光子成像能力。该设施还通过蔡司Axiophot三摄像头显微镜和新获得的蔡司活细胞成像显微镜提供常规荧光显微镜，该显微镜具有能够进行四维成像（实时3D）的最先进Apotome模块。体内小动物光学成像是一项新获得的技术，通过购买Kodak IS2000MM小动物成像仪可供核心使用。共聚焦设备的分析系统包括SiliconGraphics Octane工作站、4个Macintosh G4工作站 以及配备各种分析软件和照相品质打印机的Dell Precision 650工作站。

The purpose of the Confocal Core is to enhance the peer-reviewed funded research activities of Karmanos Cancer Institute members whose research requires confocal microscopy and related techniques. Capabilities and services include: Confocal microscopy, high resolution conventional light microscopy, in vivo small animal imaging, in situ hybridization, cytochemistry, histochemistry, immunocytochemistry, immunohistochemistry, intracellular trafficking studies, multiple labeling studies (up to 3, soon to expand to 7, different parameters can be studied at the same time) and ratiometric analyses (e.g., intracellular pH and ion measurement studies), as well as three dimensional image reconstruction and quantitative measurements. The confocal imaging system consists of a Zeiss LSM 310 confocal microscope equipped with three lasers, a Meridian InsightPlus confocal microscope equipped with one laser and the newly acquired Zeiss LSM 510 META confocal microscope with 4 lasers and multiphoton imaging capability. The facility also provides conventional fluorescent microscopy through a Zeiss Axiophot Triple-Camera Photomicroscope and the newly acquired Zeiss live cell imaging microscope with the state of the art Apotome module capable of four dimensional imaging (3D in time). In vivo small animal optical imaging is a newly acquired technology made available to the core through the purchase of the Kodak IS2000MM small animal imager. The analytical systems of the Confocal Facility include a SiliconGraphics Octane workstation, 4 Macintosh G4 workstations as well as a Dell Precision 650 workstation with various analytical software and photographic quality printers.