Microscopy, Imaging and Cytometry Resources

显微镜、成像和细胞学资源(MICR)核心的目的是增强同级 审查研究需要共焦显微镜的KCI成员的资助研究活动 细胞学，或小动物成像相关技术。功能和服务包括：Conocus 显微镜、多光子显微镜、高分辨率常规光学显微镜、FRET和FRAP，in 活体小动物成像(包括MR、PET和光学成像)、流式细胞术、完整动物和 细胞组织学服务(包括细胞化学、组织化学、免疫细胞化学 免疫组织化学)和比率分析(例如，细胞内pH和离子测量研究)，AS 以及三维和四维图像重建和定量测量。 我们的先进成像系统包括一台蔡司LSM 310共焦显微镜，配备四台激光 LINES，新收购的PerkinElmer UltraView ERS旋转盘共焦显微镜配备 三条激光谱线，蔡司LSM 510 Meta NLO共聚焦显微镜，八条激光谱线和多光子 成像能力，新收购的徕卡TCS SPS MP配备了九条激光线路和多光子 能力。我们的流式细胞仪系统包括LSR II、FACSCanto、FACSCalibur和FACScan Flow 细胞仪以及FACSDiVa细胞分选机。该设施还提供传统的荧光 蔡司三摄像头光学显微镜、蔡司活细胞成像显微镜进行显微观察 拥有最先进的Apotome模块，能够进行四维成像(时间上的3D)。活体内小 动物成像是一种新获得的技术组合，通过 增加柯达IS4000 MM小动物光学/X射线成像仪、Bruker 4.7T和11.7T小动物MR 成像仪和协和公司的MicroPET小动物PET扫描仪。MICR的分析系统包括 五个Apple和PC工作站，配备各种分析软件和照片质量打印机，以及 MIP(分子成像门户)，这是一个基于UNIX的中央服务器，存储容量为45 TB，容量为74 TB 机器人磁带备份系统以及托管高级映像分析的专用应用程序服务器 软件、MatLab和Definiens，每个许可证允许最多五个用户访问每个许可证 软件同时运行。 自2004年上次审查以来，在核心新设备和新设备方面进行了大量投资 升级成本超过280万美元。所有资金都来自CCSG以外的来源。

The purpose of the Microscopy, Imaging and Cytometry Resources (MICR) Core is to enhance the peer reviewed funded research activities of KCI members whose research requires confocal microscopy cytometry, or small animal imaging related techniques. Capabilities and services include: confocal microscopy, multiphoton microscopy, high resolution conventional light microscopy, FRET and FRAP, in vivo small animal imaging (including MR, PET and optical imaging), flow cytometry, complete animal and cellular histology services (including cytochemistry, histochemistry, immunocytochemistry immunohistochemistry) and ratiometric analyses (e.g., intracellular pH and ion measurement studies), as well as three and four dimensional image reconstruction and quantitative measurements. Our advanced imaging systems consist of a Zeiss LSM 310 confocal microscope equipped with four laser lines, the newly acquired PerkinElmer UltraView ERS spinning disk confocal microscope equipped with three laser lines, a Zeiss LSM 510 META NLO confocal microscope with eight laser lines and multiphoton imaging capability, the newly acquired Leica TCS SPS MP equipped with nine laser lines and multiphoton capability. Our flow cytometry systems include LSR II, FACSCanto, FACSCalibur and FACScan flow cytometers as well as the FACSDiVa cell sorter. The facility also provides conventional fluorescent microscopy through a Zeiss Axiophot Triple-Camera Photomicroscope, a Zeiss live cell imaging microscope with the state-of-the-art Apotome module capable of four dimensional imaging (3D in time). In vivo small animal imaging is a newly acquired combination of technologies made available to the core through the addition of a Kodak IS4000MM small animal optical/X-Ray imager, Bruker 4.7T and 11.7T small animal MR imagers and a Concord MicroPET small animal PET scanner. The analytical systems of the MICR include five Apple and PC workstations with various analytical software and photographic quality printers as well as the MIP (Molecular Imaging Portal), a UNIX based central server with 45 tb storage capacity and a 74 tb robotic tape backup system as well as a dedicated applications server hosting the advance image analysis software, Matlab¿ and Definiens¿, with five licenses each allowing up to five users access to each software simultaneously. Since the last review in 2004, there has been a substantial investment in the core for new equipment and upgrades at a cost exceeding $2,800,000. All of the funding was from sources other than the CCSG.