Microscopy, Imaging and Cytometry Resources

显微镜、成像和细胞计数资源 (MICR) 核心的目的是增强同行的能力 审查了 KCI 成员资助的研究活动，这些成员的研究需要共焦显微镜 细胞计数或小动物成像相关技术。能力和服务包括： 共聚焦 显微镜、多光子显微镜、高分辨率传统光学显微镜、FRET 和 FRAP 小动物活体成像（包括MR、PET和光学成像）、流式细胞术、完整动物和 细胞组织学服务（包括细胞化学、组织化学、免疫细胞化学 免疫组织化学）和比例分析（例如细胞内 pH 和离子测量研究），如 以及三维和四维图像重建和定量测量。 我们先进的成像系统包括配备四个激光器的 Zeiss LSM 310 共焦显微镜 新购置的 PerkinElmer UltraView ERS 转盘共焦显微镜配备了 3 条激光线，配备 8 条激光线和多光子的 Zeiss LSM 510 META NLO 共焦显微镜 成像能力方面，新购置的 Leica TCS SPS MP 配备 9 条激光线和多光子 能力。我们的流式细胞术系统包括 LSR II、FACSCanto、FACSCalibur 和 FACScan flow 细胞计数器以及 FACSDiVa 细胞分选仪。该设施还提供传统荧光灯 通过 Zeiss Axiophot 三相机光学显微镜（蔡司活细胞成像显微镜）进行显微镜检查 配备最先进的 Apotome 模块，能够进行四维成像（时间 3D）。体内小 动物成像是新获得的技术组合，通过以下方式提供给核心： 添加柯达 IS4000MM 小动物光学/X 射线成像仪、Bruker 4.7T 和 11.7T 小动物 MR 成像仪和 Concord MicroPET 小动物 PET 扫描仪。 MICR 的分析系统包括 五个 Apple 和 PC 工作站，配有各种分析软件和照相质量打印机以及 MIP（分子成像门户），一个基于 UNIX 的中央服务器，具有 45 TB 存储容量和 74 TB 机器人磁带备份系统以及托管高级图像分析的专用应用程序服务器 软件 Matlab 和 Definiens，每个都有五个许可证，最多允许五个用户访问每个许可证 软件同时进行。 自2004年上次审查以来，对新设备和新设备的核心进行了大量投资 升级费用超过 2,800,000 美元。所有资金均来自 CCSG 以外的来源。

The purpose of the Microscopy, Imaging and Cytometry Resources (MICR) Core is to enhance the peer reviewed funded research activities of KCI members whose research requires confocal microscopy cytometry, or small animal imaging related techniques. Capabilities and services include: confocal microscopy, multiphoton microscopy, high resolution conventional light microscopy, FRET and FRAP, in vivo small animal imaging (including MR, PET and optical imaging), flow cytometry, complete animal and cellular histology services (including cytochemistry, histochemistry, immunocytochemistry immunohistochemistry) and ratiometric analyses (e.g., intracellular pH and ion measurement studies), as well as three and four dimensional image reconstruction and quantitative measurements. Our advanced imaging systems consist of a Zeiss LSM 310 confocal microscope equipped with four laser lines, the newly acquired PerkinElmer UltraView ERS spinning disk confocal microscope equipped with three laser lines, a Zeiss LSM 510 META NLO confocal microscope with eight laser lines and multiphoton imaging capability, the newly acquired Leica TCS SPS MP equipped with nine laser lines and multiphoton capability. Our flow cytometry systems include LSR II, FACSCanto, FACSCalibur and FACScan flow cytometers as well as the FACSDiVa cell sorter. The facility also provides conventional fluorescent microscopy through a Zeiss Axiophot Triple-Camera Photomicroscope, a Zeiss live cell imaging microscope with the state-of-the-art Apotome module capable of four dimensional imaging (3D in time). In vivo small animal imaging is a newly acquired combination of technologies made available to the core through the addition of a Kodak IS4000MM small animal optical/X-Ray imager, Bruker 4.7T and 11.7T small animal MR imagers and a Concord MicroPET small animal PET scanner. The analytical systems of the MICR include five Apple and PC workstations with various analytical software and photographic quality printers as well as the MIP (Molecular Imaging Portal), a UNIX based central server with 45 tb storage capacity and a 74 tb robotic tape backup system as well as a dedicated applications server hosting the advance image analysis software, Matlab¿ and Definiens¿, with five licenses each allowing up to five users access to each software simultaneously. Since the last review in 2004, there has been a substantial investment in the core for new equipment and upgrades at a cost exceeding $2,800,000. All of the funding was from sources other than the CCSG.