Shared Resource: Microscopy, Imaging and Cytometry Resources

摘要 显微镜，成像和细胞计数资源（MICR）核心的目的是提高同行 审查了KCI成员资助的研究活动，他们的研究需要共聚焦显微镜，流动 流式细胞术、小动物成像和相关技术。为此，在本供资期间， 核心已经促进了92个癌症中心成员的同行评审出版物。MICR核心分为 在基础研究核心集群，其中，除了MICR核心，包括蛋白质组学和 动物模型和治疗评价核心。能力和服务包括：共聚焦显微镜， 多光子显微镜，常规光学显微镜，FRET和FRAP，TIRF和原子力显微镜， 体内小动物成像（包括SPECT、CT、PET、x射线和光学成像）、流式细胞术和 比率分析（例如，细胞内pH和离子测量研究），以及三个和四个- 三维图像重建和定量测量。磁共振成像也可通过 这是MICR和MR中心之间的合作。我们先进的成像系统包括蔡司LSM 410共聚焦显微镜，配备六条激光线，PerkinElmer UltraView ERS旋转盘共聚焦 配备三条激光线的Zeiss LSM-510 META NLO共聚焦显微镜，配备八条激光线的Zeiss LSM-510 META NLO共聚焦显微镜， Leica TCS-SP 5 MP配备了9条激光线和多光子成像功能， 新收购的奥林巴斯/布鲁克TIRF/AFM定制显微镜和尖端的蔡司LSM 780共聚焦显微镜 显微镜与六个传统的激光线和InTune激光，可调谐白色激光器，超过200 激光线。我们的流式细胞仪系统包括Becton Dickinson（BD）LSR II和BD FACSCanto II流式细胞仪。 流式细胞仪、BD FACS Vantage SE SORP细胞分选仪和新收购的Amnis ImageStreamX Mark II 成像流式细胞仪。该设施还提供了传统的荧光显微镜通过蔡司 Axiophot三摄像头显微镜，蔡司活细胞成像显微镜，具有最先进的技术水平 Apotome模块能够进行四维成像（实时3D）。体内小动物成像单元是一种 通过增加Bruker In Vivo，新升级的核心技术组合 Extreme和MS FX Pro小动物光学/X射线成像仪，Siemens Inveon SPECT/CT和Concord MicroPET R4小型动物扫描仪如上所述，小动物MRI也可通过 MICR和隔壁MR中心之间的合作努力。MICR的分析系统 核心包括八个苹果和PC工作站与各种分析软件和摄影质量 打印机和MIP，分子成像门户。MIP是一个基于UNIX的中央服务器，具有45 TB存储空间 容量和74 TB自动磁带备份系统，以及托管 高级图像分析软件，Matlab™和Esclens ™，两个许可证，每个许可证最多允许两个用户 同时访问每个软件。

ABSTRACT The purpose of the Microscopy, Imaging and Cytometry Resources (MICR) Core is to enhance the peer reviewed funded research activities of KCI members whose research requires confocal microscopy, flow cytometry, small animal imaging and related techniques. To this end, during the current funding period the Core has facilitated 92 peer-reviewed publications by the cancer center members. The MICR Core is grouped in the Basic Research Core Cluster which, in addition to the MICR Core, includes the Proteomics and the Animal Model and Therapeutics Evaluation Cores. Capabilities and services include: confocal microscopy, multiphoton microscopy, conventional light microscopy, FRET and FRAP, TIRF and Atomic Force Microscopy, in vivo small animal imaging (including SPECT, CT, PET, x-ray, and optical imaging), flow cytometry and ratiometric analyses (e.g., intracellular pH and ion measurement studies), as well as three- and four- dimensional image reconstruction and quantitative measurements. MR imaging is also available through a collaborative effort between MICR and the MR Center. Our advanced imaging systems consist of a Zeiss LSM 410 confocal microscope equipped with six laser lines, a PerkinElmer UltraView ERS spinning disk confocal microscope equipped with three laser lines, a Zeiss LSM-510 META NLO confocal microscope with eight laser lines and multiphoton imaging, a Leica TCS-SP5 MP equipped with nine laser lines and multiphoton capability, a newly acquired Olympus/Bruker TIRF/AFM custom microscope and the cutting-edge Zeiss LSM 780 confocal microscope with six conventional laser lines and the InTune laser, a tunable white light laser with over 200 laser lines. Our flow cytometry systems include Becton Dickinson (BD) LSR II and BD FACSCanto II flow cytometers, a BD FACS Vantage SE SORP cell sorter and the newly acquired Amnis ImageStreamX Mark II imaging flow cytometer. The facility also provides conventional fluorescent microscopy through a Zeiss Axiophot Triple-Camera Photomicroscope, a Zeiss live cell imaging microscope with the state-of-the-art Apotome module capable of four dimensional imaging (3D in time). The in vivo small animal imaging unit is a newly upgraded combination of technologies made available to the core through the addition of Bruker In Vivo Extreme and MS FX Pro small animal optical/x-ray imagers, Siemens Inveon SPECT/CT and Concord MicroPET R4 small animal scanners. As mentioned above, small animal MRI is also available through collaborative efforts between MICR and the MR Center located next door. The analytical systems of the MICR Core include eight Apple and PC workstations with various analytical software and photographic quality printers and the MIP, Molecular Imaging Portal. MIP is a UNIX based central server with 45 TB storage capacity and a 74 TB robotic tape backup system as well as a dedicated applications server hosting the advance image analysis software, Matlab™ and Definiens™, with two licenses each allowing up to two users access to each software simultaneously.