Microvessel Density With High Spectral/Spatial MRI

描述（由申请人提供）：MRI基于造影剂的摄取识别与恶性肿瘤中血管生成相关的致密、可渗透血管。然而，需要提高MRI的准确性。通过MRI改善血管生成的检测将对癌症的检测和治疗产生重大影响。 本实验室先前的工作表明，高光谱和空间分辨率（HiSS）MRI增加了对造影剂效应的敏感性，因为它检测到造影剂（CA）的光谱不均匀效应。CA对来自单个小体素的水共振的小成分的影响可以指示亚体素血管生成病灶的存在。拟议的研究检验了对比增强HiSS MRI增加了检测血管生成素的灵敏度和特异性的假设。我们将开发用于获取和处理HiSS数据集的方法，以最大限度地提高对造影剂的敏感性，并评估HiSS MRI是否准确检测血管生成。将评价非常小的体素中的血容量、毛细血管通透性和血管结构的HiSS MRI测量。HiSS MRI对血管结构和功能的敏感性将与高分辨率常规MRI进行比较。为了开发和评估肿瘤血管的HiSS检测，我们将：1。改进HiSS数据的采集和处理方法，优化对造影剂的灵敏度。 2.使用高分子量CA的HiSS成像测量血管体积和渗透性。 3.将HiSS数据与传统的高分辨率MRI方法进行比较。 4.将HiSS图像数据与微血管密度和血管铸型的组织学测量结果进行比较。 5.测试HiSS检测抗血管生成治疗对肿瘤血管系统的影响的能力。 该提案要求支持开发创新的MR方法，以解决重要的临床问题。创新领域是1）HiSS是MRI的新方法2）造影剂效应的HiSS检测是动态对比增强MRI的新方法。HiSS MR数据目前在临床上可接受的运行时间内使用回波平面光谱成像（EPSI）在全身扫描仪上采集。因此，拟议工作的结果可以迅速转化为临床实践，并将改善患者护理。

DESCRIPTION (provided by applicant): MRI identifies dense, permeable vasculature associated with angiogenesis in malignant tumors based on uptake of contrast agents. However, increases in the accuracy of MRI are needed. Improving detection of angiogenesis by MRI would have a significant impact on detection and treatment of cancer. Previous work in this laboratory showed that high spectral and spatial resolution (HiSS) MRI increases sensitivity to the effects of contrast agents because it detects spectrally inhomogeneous affects of contrast agents (CA's). CA effects on small components of the water resonance from single small voxels may indicate the presence of sub-voxelar angiogenic foci. The proposed research tests the hypothesis that contrast enhanced HiSS MRI has increased sensitivity and specificity for detection of ang/ogenes/s. We will develop methods for acquiring and processing HiSS datasets to maximize sensitivity to contrast media and evaluate whether HiSS MRI accurately detects angiogenesis. HiSS MRI measurements of blood volume, capillary permeability, and vascular architecture in very small voxels will be evaluated. Sensitivity of HiSS MRI to vascular structure and function will be compared to that of high-resolution conventional MRI. To develop and evaluate HiSS detection of tumor vasculature we will: 1. Improve methods for acquiring and processing HiSS data that optimize sensitivity to contrast agents. 2. Measure vascular volume and permeability using HiSS imaging of high molecular weight CA's. 3. Compare HiSS data with conventional high-resolution MRI methods. 4. Compare HiSS image data to histological measurements of micro-vessel density and vascular casts. 5. Test the ability of HiSS to detect effects of anti-angiogenic therapy on tumor vasculature. This proposal requests support for development of innovative MR methods that attack an important clinical problem. The areas of innovation are 1) HiSS is a new approach to MRI 2) HiSS detection of effects of contrast agents is a novel approach to dynamic contrast enhanced MRI. HiSS MR data are currently acquired in clinically acceptable run-times on whole-body scanners using echo-planar-spectroscopic imaging (EPSI). Thus, the results of the proposed work could be rapidly translated to clinical practice and would improve patient care.