Coordination of localized actin polymerization with endosomal membrane dynamics

为了使细胞对环境做出反应，它们必须使用几种途径将分子移动到细胞内的正确目的地，在那里可以处理信号，并且可以调整它们的行为。一旦分子被内化，它们就从分选室被分选到不同的途径中。然而，细胞如何能够识别和分选分子进入正确的途径仍不清楚。最近的证据表明，WASH产生的肌动蛋白网络在这些分类隔室的功能。使用低温相关成像方法，我们将能够以一种新的视角观察这个系统，以深入了解WASH及其肌动蛋白网络在这个分选过程中的参与。这将提高我们对细胞信号传导以及细胞如何适应环境线索的理解，这将是至关重要的，因为信号传导过程中的错误是导致许多病理状况的原因。此外，我们的工作还包括开发优化的低温相关成像设备和评估新兴成像技术的程序。

For cells to respond to their environment, they must employ several pathways to move molecules to the correct destination within the cell where signals can be processed, and their behaviour can be adjusted in response. Once molecules are internalized, they are sorted into different pathways from sorting compartments. However, how the cell is capable of recognizing and sorting molecules into the correct pathways remains unclear. Recent evidence implicates the WASH generated actin network in the function of these sorting compartments. Using a cryogenic correlative imaging approach, we will be able to observe this system with a novel perspective to gain insight into the involvement of WASH and its actin network in this sorting process. This will improve our understanding of cell signalling and how cells adapt in response to environmental cues, which will be essential as errors in signalling processes are responsible for numerous pathological conditions. Additionally, our work includes a component of development of optimized cryogenic correlative imaging equipment and procedures for the evaluation of emerging imaging technology.