Development Of A Vaccine For Leishmania Major Infection

开发利什曼原虫重大感染疫苗

• 批准号: 6987266
• 负责人: ROBERT A SEDER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6987266
• 关键词: CpG islands; Leishmania major; Primates; active immunization; biotechnology; cellular immunity; cytokine; cytotoxic T lymphocyte; dosage; enzyme linked immunosorbent assay; helper T lymphocyte; histopathology; immune response; interleukin 12; laboratory mouse; leishmaniasis; nonhuman therapy evaluation; protozoal antigen; protozoal vaccine; recombinant virus; vaccine development; vaccine evaluation; vector vaccine

Infection with Leishmania causes significant morbidity and mortality worldwide. The type of Leishmania species infecting the host and the immune response generated by the host determines the spectrum of clinical disease that is seen. In particular, the pattern of cytokine production from T cells is critical for protection. At present there is no vaccine for Leishmania that is easy to administer, efficacious, and cost effective. These experiments will seek to establish a vaccine regimen that is sufficient to confer long-term protective immunity following infectious challenge in mice and primates. Experiments will specifically focus on DNA, protein and recombinant viral vaccines. In addition, these studies will evaluate the cellular and molecular mechanisms by which different vaccine formulations induce long-term protective cellular immunity.

利什曼原虫感染在世界范围内引起显著的发病率和死亡率。感染宿主的利什曼原虫种类和宿主产生的免疫应答决定了所观察到的临床疾病谱。特别是，T细胞产生细胞因子的模式对于保护至关重要。目前还没有一种易于管理、有效且具有成本效益的利什曼原虫疫苗。这些实验将寻求建立一种疫苗方案，该方案足以在小鼠和灵长类动物感染性攻击后赋予长期保护性免疫力。实验将特别侧重于DNA、蛋白质和重组病毒疫苗。此外，这些研究将评估不同疫苗制剂诱导长期保护性细胞免疫的细胞和分子机制。