Development of a platform for tracking the stability of advanced BioTherapeutics

开发用于跟踪先进生物疗法稳定性的平台

• 批准号: 2605111
• 金额: --
• 依托单位: University of Bath
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2605111
• 关键词: Development; platform; tracking; stability; advanced

Biopharmaceutical stability has been a critical concern as drugs are being taken off the market due to safety issues with little to no effort in replacing them. Hence, with the aim of understanding the stability issues surrounding large biologics, this project involves monitoring the REES effect under various factors affecting stability. Red Edge Excitation Shift (REES) is a fluorescence method that is obtained by exciting the fluorophore towards the red edge of the visible light spectrum and detecting the fluorescence emission from the fluorophore. In doing so one can gain insight into protein dynamics and structure. Various biopharmaceuticals will be measured such as viral vectors, exosomes and monoclonal antibodies using a fluorescence spectrometer. The results will thus be further supported using orthogonal methods such as AFM, TEM, DLS and DSC to verify the findings of REES.

生物制药的稳定性一直是一个关键问题，因为药物由于安全问题而退出市场，几乎没有努力取代它们。因此，为了了解大型生物制品的稳定性问题，该项目涉及在影响稳定性的各种因素下监测REES效应。红边激发位移（REES）是一种荧光方法，其通过朝向可见光谱的红边激发荧光团并检测来自荧光团的荧光发射来获得。通过这样做，人们可以深入了解蛋白质的动力学和结构。将使用荧光光谱仪测量各种生物药物，如病毒载体、外来体和单克隆抗体。因此，结果将进一步支持使用正交方法，如AFM，TEM，DLS和DSC，以验证REES的发现。