Fourier Transform Mass Spec Proteomics and Genomics

傅里叶变换质谱蛋白质组学和基因组学

• 批准号: 6804239
• 负责人: Ian Alexander Blair
• 金额: $ 77.16万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6804239
• 关键词: biomedical equipment purchase; functional /structural genomics; ion cyclotron resonance spectrometry; mass spectrometry; proteomics; structural biology

DESCRIPTION (provided by applicant): Fourier Transform Mass Spectrometry for Proteomics and Genomics: Fourier transform ion cyclotron resonance-mass spectrometry (FTICR-MS) instruments are relatively new to proteomics and genomics research. FTICR/MS rapidly provides highly accurate mass determinations of peptides derived from protease digests of intact proteins. Thus, FTICR-MS systems provide unparalleled capabilities for rapid fingerprinting of known proteins as well as the characterization of new proteins. They can also be used for the unequivocal characterization of covalently modified DNA. Thus, such instruments are essential for state-of-the-art of research in the area of proteomics research when only trace amounts of material are available. The Thermo Finnigan system (LTQ FT) can utilize a variety of ionization techniques: ESI, APCI, APPI or MALDI. Maintenance of the API source, as well as switching between AP ionization methods, is vent-free. Ions are transferred by octapole and "square" quadrupole lenses into a novel ion trap. The linear ion trap is a fully operational mass spectrometer, which can store, isolate, and fragment ions and then send them either to the ICR cell via axial ion ejection for further analysis or to an off-axis SEM detector. The linear ion trap is a unique ion preparation and injection system for FTICR-MS because it has much greater ion storage capacity than conventional 3D ion trap devices. Ions from the ion trap are transferred as discrete bundles into the ICR cell, via octapole ion guides. These octapole ion guides are designed to enable very efficient differential pumping between the ion trap and the ICR cell. The FTICR analyzer is built around an actively shielded superconducting magnet with a field strength of 7 Tesla. The investigators involved in this proposal have research programs that represent four major research areas within the University of Pennsylvania. These programs are highly interactive. Dr. Blair as Director of the Center for Cancer Pharmacology and Scientific Director of the Genomics Institute Proteomics Resource is a member the Cancer Center, the Center for Experimental Therapeutics, and the Institute for Medicine and Engineering. Dr. FitzGerald as Director of the Center for Experimental Therapeutics and a member of the Institute for Medicine and Engineering has extensive interactions with Dr. Davies, Director of the Institute for Medicine and Engineering and Dr. Rader, Director, Lipid Clinics; Assistant Director, General Clinical Research Center through an existing program project grant and a SCOR grant. Three additional investigators from these research programs (Drs. Eberwine, Ischiropoulos, and Roos) will require access to the instrument on a more limited basis. The proposed research on the two major degenerative disease of aging, cancer and cardiovascular disease, will involve a highly interactive approach. This will permit advances made by one investigator to be readily incorporated into research of the other investigators. Thus, while each investigator's research proposal stands alone in scientific value and integrity, the added value of this combined proposal is substantial.

描述（由申请人提供）： 蛋白质组学和基因组学的傅立叶变换质谱法：傅立叶转化离子回旋共振 - 质谱法（FTICR-MS）仪器在蛋白质组学和基因组学研究中相对较新。 FTICR/MS迅速提供了从完整蛋白质蛋白酶消化中得出的肽的高度准确的质量测定。因此，FTICR-MS系统为已知蛋白质的快速指纹以及新蛋白质的表征提供了无与伦比的功能。它们也可以用于共价修饰的DNA的明确表征。因此，当仅痕量材料可用时，此类仪器对于蛋白质组学研究领域的最新研究至关重要。 Thermo Finnigan系统（LTQ FT）可以利用各种电离技术：ESI，APCI，APPI或MALDI。 API源的维护以及在AP电离方法之间切换，不含通风孔。离子由八杆酮和“正方形”四极透镜转移到一个新型离子陷阱中。线性离子陷阱是一个完全操作的质谱仪，可以存储，隔离和碎片离子，然后通过轴向离子射血将其发送到ICR细胞，以进行进一步分析或转移到离轴SEM检测器上。线性离子陷阱是FTICR-MS的独特离子制备和注入系统，因为它的离子存储容量比常规的3D离子陷阱设备要大得多。来自离子陷阱的离子通过八极离子指南将离散束作为离散束转移到ICR细胞中。这些八极离子指南旨在实现离子陷阱和ICR电池之间非常有效的差分泵。 FTICR分析仪围绕着一个主动屏蔽的超导磁铁，场强为7特斯拉。参与该提案的研究人员的研究计划代表了宾夕法尼亚大学的四个主要研究领域。这些程序是高度互动的。 Blair博士担任癌症药理学中心主任和基因组学院蛋白质组学资源的科学总监是癌症中心，实验治疗中心和医学与工程研究所。 Fitzgerald博士是实验治疗中心主任，医学与工程研究所的成员与医学与工程研究所主任Davies博士以及脂质诊所主任Rader博士进行了广泛的互动；通过现有计划项目赠款和SCOR赠款，通用临床研究中心助理主任。这些研究计划（Eberwine博士，Ischiropoulos和Roos）的另外三名研究人员将需要更有限的基础上使用该乐器。关于衰老，癌症和心血管疾病的两种主要退化性疾病的拟议研究将涉及一种高度互动的方法。这将使一名研究人员的进步很容易被纳入其他研究人员的研究中。因此，尽管每个研究者的研究建议独自以科学价值和完整性为单独，但该组合提案的附加值却是重要的。