Affymetrix Gene Chip Node System 3000

Affymetrix基因芯片节点系统3000

• 批准号: 6731649
• 负责人: John Barker Harley
• 金额: $ 19.9万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731649
• 关键词: biomedical equipment purchase; microarray technology

DESCRIPTION (provided by applicant): An Affymetrix(r) Gene Chip/R Node System is requested for the central equipment facilities of the Oklahoma Medical Research Foundation (OMRF). The requested instruments will provide investigators with access to the high standard of quality available though the Affymetrix technology for gene expression experiments, large resequencing projects, and human genome scan single nucleotide polymorphisms. In addition, the well-known Affymetrix technology provides the capacity to very rapidly re-sequence up to 30,000 bases of DNA for variants at low cost and at very high reliability. Finally, Affymetrix has developed arrays that permit the determination of alleles at a large number of genomic sites containing single nucleotide polymorphisms (SNPs). The capability now is at approximately 10,000 SNPs per array and is planned to be >100,000 SNPs in 2004, when this instrument would first be available to us. Major investigator user groups at the OMRF and at the University of Oklahoma will use at least 85% of the capacity of this instrument and its accompanying Affymetrix technology for gene expression, for re-sequencing, and for genotyping. This capability will have a substantial impact upon a number of NIH-funded projects now underway, especially by providing technical capacity not now available to them. The two gene array systems now available on this campus support a number of investigators with less expensive and less reliable spotted arrays for gene expression array data collection. Neither of these facilities has comparable genotyping or re-sequencing or SNP genotyping capabilities. Moreover, we suspect that one of the major applications of the requested equipment will be to confirm and extend gene expression results obtained by other less expensive technologies. The equipment package requested includes an array reader, a computer workstation, a fluidics station, and a hybridization station, multiple training sessions, easy access to technical assistance, and maintenance for the first year. The expense for support for instrument use and the maintenance contracts in future years will be borne by the OMRF.

描述（由申请人提供）： 俄克拉荷马医学研究基金会 (OMRF) 的中央设备设施需要 Affymetrix(r) 基因芯片/R 节点系统。所需的仪器将为研究人员提供通过 Affymetrix 技术进行基因表达实验、大型重测序项目和人类基因组扫描单核苷酸多态性的高质量标准。此外，众所周知的 Affymetrix 技术能够以低成本和极高的可靠性对多达 30,000 个 DNA 碱基的变异进行快速重新测序。最后，Affymetrix 开发了阵列，可以测定大量含有单核苷酸多态性 (SNP) 的基因组位点的等位基因。现在每个阵列的能力约为 10,000 个 SNP，并计划在 2004 年首次向我们提供该仪器时达到 >100,000 个 SNP。 OMRF 和俄克拉荷马大学的主要研究用户组将使用该仪器及其随附的 Affymetrix 技术的至少 85% 的容量来进行基因表达、重新测序和基因分型。这种能力将对美国国立卫生研究院资助的许多正在进行的项目产生重大影响，特别是通过提供他们现在无法获得的技术能力。该校园现在提供的两个基因阵列系统支持许多研究人员使用更便宜且不太可靠的斑点阵列来收集基因表达阵列数据。这些设施均不具备可比的基因分型、重测序或 SNP 基因分型能力。此外，我们怀疑所需设备的主要应用之一将是确认和扩展通过其他较便宜的技术获得的基因表达结果。所需的设备包包括阵列读取器、计算机工作站、流体站和杂交站、多个培训课程、轻松获得技术援助以及第一年的维护。未来几年仪器使用支持和维护合同的费用将由 OMRF 承担。