Gene Expression Array Scanner & Support Instrumentation

基因表达阵列扫描仪

• 批准号: 6731361
• 负责人: MARTINA L VEIGL
• 金额: $ 35.39万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731361
• 关键词: biomedical equipment purchase; high throughput technology; microarray technology

DESCRIPTION (provided by applicant): This proposal seeks funds to purchase instrumentation that will allow the Case Western Reserve Cancer Center Gene Expression Array Core Facility [GEACF] to maintain state-of-the art services for its research community, while simultaneously addressing rapidly increasing demands for high sample number throughput and data reduction. To accomplish this aim, this proposal requests funds for an instrument package to be added to the existing Case Western Reserve University [CWRU] Core Facility and will provide the following: a) A new Affymetrix GeneChip Microarray Scanner System to allow experimental access to the new generation of gene expression microarrays; b) A system to improve the assessment of sample quality to facilitate the utilization of samples from translational studies and c) A state-of-the-art data management package tailored to address the data management needs of moderate to high-throughput laboratories. The ability to assess gene expression using microarrays is an essential resource in modern biomedical research. The Cancer Center at CWRU and University Hospitals of Cleveland [UHC] established the GEACF in January 2000, to make this technology available to their scientific investigators. The full service Affymetrix technology-based GEACF offers technical assistance and advice on all aspects of gene-expression technology. Over the past 3 years, the GEACF has rapidly grown from a Core Facility established to address the needs of Cancer Center investigators to a Facility that processes samples for CWRU, its Medical School and its affiliated institutions [UHHS, MetroHealth and The V.A. Hospital] as well as for the research faculty of the Cleveland Clinic Foundation. The GEACF supports diverse research by processing samples harvested from tissue culture, animals and people. Species currently emphasized include yeast, mouse, rat and human. The Core Facility currently supports the research efforts of 79 principal investigators. However, over the next 2 years we anticipate a significant expansion of our User base. In this regard, the CWRU Medical School is completing a large building project over the coming year, which will bring on-line more than 222,000 sq. ft. of additional laboratory space. The GEACF Facility will play a significant role in providing research infrastructure for researchers recruited to this new facility and investigators using its animal resources center that will house more than 70,000 mice.

描述（由申请人提供）：该提案寻求资金购买仪器，使凯斯西储癌症中心基因表达阵列核心设施 [GEACF] 能够为其研究界维持最先进的服务，同时满足快速增长的高样本量吞吐量和数据减少的需求。为了实现这一目标，本提案要求为现有凯斯西储大学 [CWRU] 核心设施添加仪器包提供资金，并将提供以下内容： a) 新的 Affymetrix GeneChip 微阵列扫描仪系统，允许实验访问新一代基因表达微阵列； b) 改进样品质量评估的系统，以促进转化研究样品的利用；以及 c) 专为满足中高通量实验室的数据管理需求而定制的最先进的数据管理包。 使用微阵列评估基因表达的能力是现代生物医学研究的重要资源。 CWRU 癌症中心和克利夫兰大学医院 [UHC] 于 2000 年 1 月成立了 GEACF，以便向其科学研究人员提供这项技术。基于 Affymetrix 技术的 GEACF 全方位服务提供基因表达技术各个方面的技术援助和建议。在过去 3 年中，GEACF 已从一个为满足癌症中心研究人员的需求而建立的核心设施迅速发展成为为 CWRU、其医学院及其附属机构 [UHHS、MetroHealth 和 V.A.医院]以及克利夫兰诊所基金会的研究人员。 GEACF 通过处理从组织培养物、动物和人身上采集的样本来支持多样化的研究。目前重点关注的物种包括酵母、小鼠、大鼠和人类。核心设施目前支持 79 名主要研究人员的研究工作。然而，在接下来的两年里，我们预计我们的用户群将显着扩大。在这方面，CWRU 医学院将于明年完成一项大型建筑项目，这将带来超过 222,000 平方英尺的额外实验室空间。 GEACF 设施将发挥重要作用，为新设施招募的研究人员和使用其动物资源中心的研究人员提供研究基础设施，该中心将容纳 70,000 多只小鼠。