Integrated Genomics Shared Resource

整合的基因组学共享资源 项目总结/摘要 综合基因组学共享资源（Genomics SR）是Case Comprehensive的重要资产。 癌症中心（案例CCC）成员的研究，说明了科学计划参与的广度， 仅去年一年，就有230多名研究人员使用了这一SR，其中36%的人使用了这一SR。 在使用的所有7个案例CCC程序中，案例CCC成员占总使用量的65 基因组学SR的使命是实现高通量基因组学技术的稳健使用， 癌症研究项目的数据分析。该多站点SR由Case共同支持和管理 CCC，凯斯西储大学（CWRU）医学院（SOM）和克利夫兰诊所（CC） 勒纳研究所（LRI）。它提供了一个全方位的基因组学技术，包括多， 用于DNA和RNA分析的下一代测序（NGS）站点。Genomics SR的具体目标是 收件人： 1.提供项目级基因组学咨询服务，协助研究者进行实验设计， 技术选择，关键资源的组装，以执行项目，收集样本，确保整体 项目质量控制，并确定最佳分析方法。 2.提供全方位的基因组技术，包括： a）样品制备和样品质量评估服务：从困难样品中进行核酸分离; 用于下游分析的核酸质量评价。 B）全面的基因组学分析：基于RNA的服务：RNA-seq、定制Illumina RNA面板、RNA测序等。 使用Affyssin微阵列技术进行表达分析，实时PCR;基于DNA的服务：下一页- 代测序（全基因组和外显子组定制，扩增子测序，微生物组分析， CRISPR验证，ChIP-Seq分析）;甲基化分析，桑格测序和基因分型 （TaqMan测定，微阵列）。 3.提供一系列的基因组学分析支持，包括获取基因组学分析的机会和培训 软件;以及执行高端分析，包括开发特定基因组学管道 项目和分析经验有限的调查人员。 在当前的资助周期（2012-2016年），Genomics SR为479名注册用户提供了服务， 其中134人（28%）是案例CCC成员，代表所有7个案例CCC计划。 最后，Genomics SR认为，快速访问一个重点突出、执行良好的数据分析管道对于 研究人员基因组研究的成功为此，Genomics SR开发了一个分析 组件，它确保每个项目都有强大的实验设计和数据分析计划， 入会仪式

INTEGRATED GENOMICS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Integrated Genomics Shared Resource (Genomics SR) is an essential asset to Case Comprehensive Cancer Center (Case CCC) members' research, illustrated by the breadth of scientific program involvement and reflected in the large number of investigators who use this SR. Last year alone, over 230 investigators, 36% of whom were Case CCC members, accounting for 65% of total usage, from all 7 of the Case CCC Programs used the SR. The mission of the Genomics SR is to enable robust use of high-throughput genomics technologies and data analysis for cancer research projects. This multi-site SR is jointly supported and managed by the Case CCC, the Case Western Reserve University (CWRU) School of Medicine (SOM), and Cleveland Clinic (CC) Lerner Research Institute (LRI). It provides access to a full spectrum of genomics technologies including multi- site Next Generation Sequencing (NGS) for DNA and RNA analyses. The Specific Aims of the Genomics SR are to: 1. Provide project-level genomics consultation services to assist investigators in experimental design, technology selection, assembling of key resources to execute projects, collect samples, assure overall project quality control, and identify best analytical approaches. 2. Provide access to a full range of genomic technologies, including: a) Sample preparation & sample quality assessment services: Nucleic Acid Isolation from difficult samples; Evaluation of nucleic acid quality for downstream analysis. b) Comprehensive genomics analyses: RNA-based services: RNA-seq, custom Illumina RNA panels, RNA expression analysis using Affymetrix microarray technology, real-time PCR; DNA-based services: Next- Generation Sequencing (whole genome, and -exome custom, amplicon sequencing, microbiome analysis, CRISPR validation, ChIP-Seq analysis); methylation analysis, Sanger sequencing and genotyping (TaqMan Assays, Microarrays). 3. Provide a range of genomics analysis support including access to and training on genomics analysis software; and execution of high-end analysis including development of genomics pipelines for specific projects and for investigators with limited analysis experience. Throughout the current funding cycle (2012-2016), the Genomics SR provided services to 479 registered users, 134 (28%) of whom are Case CCC members, representing all 7 of the Case CCC programs. Finally, the Genomics SR feels that rapid access to a focused, well-executed data analysis pipeline is critical to the success of investigators’ genomic studies. To this end, the Genomics SR developed an Analytics Component, which ensures that each project has robust experiment design and data analysis plans before initiation.