Affymetrix GCS3000 Instrument System

Affymetrix GCS3000 仪器系统

• 批准号: 6730921
• 负责人: Nadereh Jafari
• 金额: $ 19.93万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730921
• 关键词: biomedical equipment purchase; fluorescent in situ hybridization; gene expression profiling; microarray technology; nucleic acid sequence

DESCRIPTION (provided by applicant): The ability to use microarray-based gene profiling approaches to define global patterns of gene expression during embryonic development, in response to specific hormonal and physiological stimuli, and in pathological and disease states, in genetically modified animals, and pharmacogenomics represents an important technological advance. This technology requires both expensive and sophisticated instrumentation and specific expertise in the form of array fabrication, bioinformatics and statistical support. Recognizing the importance of this technology in biological research, two years ago Northwestern University created the Northwestern University Microarray Core (NUMAC) to provide such a resource for its investigators. Currently, NUMAC is providing a wide range of services to its users including consultation in experimental design and analysis, performing hybridizations for custom and prefabricated arrays (Affymetrix GeneChips), and printing custom arrays. The NUMAC labeling and processing service for GeneChips has been offered in conjunction with Children's Memorial Hospital Institute for Education and Research (CMIER). The number of NUMAC users has increased significantly and this has caused a long turnaround time for processing of the samples at CMIER. This problem is going to exacerbate as both NU campuses (Evanston, Chicago) employ more GeneChips related projects. The Affymetrix GeneChips can only be processed by the Affymetrix system and the goal of this application is to request funds to purchase an Affymetrix GCS Instrument to enhance our existing capabilities for the GeneChip processing in a timely manner with a higher quality.

描述（由申请人提供）：能够使用基于微阵列的基因分析方法来定义胚胎发育过程中基因表达的全局模式，以响应特定的激素和生理刺激，在病理和疾病状态下，在转基因动物和药物基因组学中，这代表了一项重要的技术进步。这项技术既需要昂贵而复杂的仪器，也需要阵列制造、生物信息学和统计支持方面的专业知识。认识到这项技术在生物研究中的重要性，两年前西北大学创建了西北大学微阵列核心（NUMAC），为其研究人员提供这样的资源。目前，NUMAC正在为其用户提供广泛的服务，包括实验设计和分析咨询，为定制和预制阵列（Affymetrix GeneChips）进行杂交，以及打印定制阵列。基因芯片的NUMAC标签和加工服务是与儿童纪念医院教育和研究所（CMIER）联合提供的。NUMAC用户的数量显著增加，这导致CMIER处理样品的周转时间较长。随着纽大的两个校区（埃文斯顿和芝加哥）采用更多与基因芯片相关的项目，这个问题将会加剧。Affymetrix基因芯片只能由Affymetrix系统处理，本申请的目的是请求资金购买Affymetrix GCS仪器，以提高我们现有的基因芯片处理能力，及时提高质量。