CORE--MORPHOLOGY CORE

核心--形态核心

• 批准号: 6884956
• 负责人: SAMUEL William FRENCH
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6884956
• 关键词: alcoholic liver cirrhosis; animal tissue; biomedical facility; cytokine; electron microscopy; histology; immunocytochemistry; immunofluorescence technique; immunoperoxidase; liver; macrophage; morphology; nuclear factor kappa beta; pancreas; sectioning; tissue /cell preparation

The main objective of the Morphology Core is to support the Center projects with high-quality and specialized morphological services while achieving net cost effectiveness. The most common types of services to be offered are classified into three categories: 1) standard staining of sections following paraffin embedding of tissues (e.g., hematoxylin and eosin, Sirius red for collagen); 2) special immunohistochemistry for fixed or frozen sections (e.g., inflammatory cells, cytokines, NF-kappaB); and 3) image and morphometric analysis to provide standardized quantitative data. In addition, the Core performs electron microscopic examination of liver and pancreas to identify non-parenchymal liver cells (hepatic and pancreatic stellate cells) or ultrastructural alterations of parenchymal liver and pancreatic acinar cells. Samples that are handled by the Core include liver (Research Project #1,2,3) and pancreas (Research Project #4) tissues; primary cultures of hepatocytes (Research Project #2) and hepatic macrophages (Research Project #3); and isolated pancreatic acini (Research Project #1). Standard and special staining procedures are carried out on these samples after preparation of frozen sections, fixation and embedding, cutting, and mounting on slides. In the case of cell cultures, the cells on chamber slides or cover slips are permeabilized and immunostained. Immunofluorescent or immunoperoxidase staining is performed using specific antibodies against the molecules of interest. Morphometric analysis is performed for quantification of histologic assessment, immunostained proteins or cells by digital imaging analysis using a digital camera and the Nikon Image Analysis system. Quality control is maintained at the Core site, Anatomic Pathology at Harbor-UCLA Medical Center, that is annually inspected and certified by the College of American Pathology and is under the directorship of the Core Director. During the past 4 years, the Core has served 9 center investigators and 2 non-center investigators. The total number of samples processed by the Core has increased from 624 in 1999 to 2,133 in 2002. It supported the Center research and pilot projects resulting in 29 publications with morphological findings. The Morphology Core continues to make a major contribution to a growth of the center-supported research.

Morphology Core的主要目标是以高质量和 专业的形态服务，同时实现净成本效益。提供的最常见的服务类型分为三类：1）组织石蜡包埋后切片的标准染色（例如，苏木精和伊红，天狼星红用于胶原）; 2）用于固定或冷冻切片的特殊免疫组织化学（例如，炎性细胞、细胞因子、NF-κ B）;和3）图像和形态测定分析以提供标准化的定量数据。此外，Core还对肝脏和胰腺进行电子显微镜检查，以识别非实质性肝细胞（肝脏和胰腺星状细胞）或实质性肝细胞和胰腺腺泡细胞的超微结构改变。的样本 由核心处理的包括肝脏（研究项目#1、2、3）和胰腺（研究项目#4）组织;肝细胞（研究项目#2）和肝巨噬细胞（研究项目#3）的原代培养物;以及分离的胰腺腺泡（研究项目#1）。在制备冷冻切片、固定和包埋、切割和封片后，对这些样本进行标准和特殊染色程序。在细胞培养的情况下，室载玻片或盖玻片上的细胞被透化和免疫染色。免疫荧光或免疫过氧化物酶染色使用针对感兴趣分子的特异性抗体进行。进行形态测定分析以定量组织学评估， 通过使用数码相机和Nikon图像分析系统的数字成像分析，对免疫染色的蛋白质或细胞进行分析。质量控制在Harbor-UCLA医学中心的解剖病理学核心中心进行，该中心每年由美国病理学学院进行检查和认证，并由核心总监负责。在过去的4年中，核心已服务于9个中心研究者和2个非中心研究者。核心小组处理的样本总数从1999年的624个增加到2002年的2 133个。它支持该中心的研究和试点项目，导致29个出版物的形态研究结果。形态学核心继续为中心支持的研究的增长做出重大贡献。