CORE--NEUROPATHOLOGY AND MOLECULAR GENETICS

核心--神经病理学和分子遗传学

• 批准号: 6798011
• 负责人: HARRY V. VINTERS
• 金额: $ 18.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6798011
• 关键词: Alzheimer' s disease; DNA; biomedical facility; body fluids; cryopreservation; dementia; gene expression; human genetic material tag; human tissue; immunocytochemistry; microarray technology; molecular genetics; neuropathology; postmortem; protein quantitation /detection; tissue /cell preparation; tissue resource /registry

The newly reconfigured Neuropathology and Molecular Genetics (NPMG) Core of the UCLA AIzheimer's Disease Research Center (ADRC) will perform autopsies and genetic studies on patients with neurodegenerative diseases (and appropriate controls) and will serve as a tissue/fluid and DNA resource for individuals performing innovative research on Alzheimer's disease (AD) and non-AD dementias. The specific aims of the Core are to carry out comprehensive postmortem examinations; harvest and store relevant tissues and fluids; assess lesions semi-quantitatively using special stains and immunohistochemistry; provide definitive diagnoses; prepare tissue microarrays for protein expression studies; expand the genetic resource to include apolipoprotein E (apoE) determinations, DNA storage, and preparation of cDNA on patients with known or suspected mutations; and support other Cores and Projects. Project 2 will use brain tissue from the NPMG Core. The autopsy and tissue harvesting/sampling method has evolved and been optimized in conjunction with interactions with the National Alzheimer's Coordinating Center (NACC). Genetic and autopsy information will be maintained in the ADRC database and transmitted to the ARCC database. The Core has performed comprehensive autopsies on 98 patients characterized in Clinical Core; and supplies tissue to investigators locally and nationally. NPMG Core interacts with Clinical Core to provide autopsy confirmation of clinical diagnoses; with Imaging Core to supply autopsy confirmed diagnoses with patients studied in neuroimaging; with the Data Management and Statistics Core through the ADRC database; with the Education/Information Transfer Core through monthly clinicopathological conferences and other educational activities. NPMG Core accomplished its specific aims in the past funding period and has added innovative new technologies such as the "molecular autopsy" approach involving routine use of microarray techniques to study gene and protein expression in appropriate autopsy cases.

UCLA的新重新配置的神经病理学和分子遗传学（NPMG）核心 Aizheimer疾病研究中心（ADRC）将对神经退行性疾病（以及适当的对照）患者进行尸检和遗传研究，并将作为对阿尔茨海默氏病（AD）和非AD痴呆症进行创新研究的个体的组织/液体和DNA资源。核心的具体目的是进行全面的验尸检查；收获并存储相关的组织和流体；使用特殊污渍和免疫组织化学评估病变半量化；提供明确的诊断；准备组织微阵列进行蛋白质表达研究；将遗传资源扩展到包括载脂蛋白E（APOE）测定，DNA储存以及对已知或疑似突变患者的cDNA制备；并支持其他核心和项目。项目2将使用NPMG核心的脑组织。与国家阿尔茨海默氏症协调中心（NACC）的相互作用，尸检和组织收集/采样方法已进化和优化。遗传和尸检信息将被维护 在ADRC数据库中，并传输到ARCC数据库。核心已执行 对98名以临床核心为特征的患者的全面尸检；并将组织提供给 调查人员在当地和全国范围内。 NPMG核心与临床核心相互作用以提供尸检 确认临床诊断；通过成像核供应尸检，确认了在神经影像学中研究的患者的诊断；通过ADRC数据库的数据管理和统计核心；教育/信息通过每月的临床病理学会议和其他教育活动通过教育/信息转移核心。 NPMG核心在过去的资金期间实现了其特定目标，并增加了创新的新技术，例如“分子尸检”方法，涉及在适当的尸检病例中进行微阵列技术的常规使用来研究基因和蛋白质表达。