CORE--NEUROPATHOLOGY AND MOLECULAR GENETICS

新重新配置的UCLA神经病理学和分子遗传学（NPMG）核心 阿尔茨海默病研究中心（ADRC）将对患有神经退行性疾病（和适当的对照）的患者进行尸检和遗传研究，并将作为对阿尔茨海默病（AD）和非AD痴呆进行创新研究的个人的组织/液体和DNA资源。核心的具体目标是进行全面的死后检查;收集和储存有关的组织和体液;使用特殊染色剂和免疫组织化学对病变进行半定量评估;提供明确的诊断;为蛋白质表达研究准备组织微阵列;扩大遗传资源，包括载脂蛋白E测定、DNA储存和对已知或怀疑有突变的病人准备cDNA;并支持其他核心和项目。项目2将使用来自NPMG核心的脑组织。尸检和组织采集/采样方法已经发展并与国家阿尔茨海默氏症协调中心（NACC）的相互作用进行了优化。遗传和尸检信息将被保留 在ADRC数据库中，并传输到ARCC数据库。核心执行 对98名患者进行了全面的尸检，这些患者在临床核心中表现出特征;并为 当地和全国的调查人员。NPMG核心与临床核心互动，以提供尸检 临床诊断的确认;影像学核心为神经影像学研究的患者提供尸检确认的诊断;通过ADRC数据库提供数据管理和统计核心;通过每月的临床病理学会议和其他教育活动提供教育/信息传输核心。NPMG Core在过去的资助期内实现了其特定目标，并增加了创新的新技术，如“分子尸检”方法，包括常规使用微阵列技术研究适当尸检病例中的基因和蛋白质表达。

The newly reconfigured Neuropathology and Molecular Genetics (NPMG) Core of the UCLA AIzheimer's Disease Research Center (ADRC) will perform autopsies and genetic studies on patients with neurodegenerative diseases (and appropriate controls) and will serve as a tissue/fluid and DNA resource for individuals performing innovative research on Alzheimer's disease (AD) and non-AD dementias. The specific aims of the Core are to carry out comprehensive postmortem examinations; harvest and store relevant tissues and fluids; assess lesions semi-quantitatively using special stains and immunohistochemistry; provide definitive diagnoses; prepare tissue microarrays for protein expression studies; expand the genetic resource to include apolipoprotein E (apoE) determinations, DNA storage, and preparation of cDNA on patients with known or suspected mutations; and support other Cores and Projects. Project 2 will use brain tissue from the NPMG Core. The autopsy and tissue harvesting/sampling method has evolved and been optimized in conjunction with interactions with the National Alzheimer's Coordinating Center (NACC). Genetic and autopsy information will be maintained in the ADRC database and transmitted to the ARCC database. The Core has performed comprehensive autopsies on 98 patients characterized in Clinical Core; and supplies tissue to investigators locally and nationally. NPMG Core interacts with Clinical Core to provide autopsy confirmation of clinical diagnoses; with Imaging Core to supply autopsy confirmed diagnoses with patients studied in neuroimaging; with the Data Management and Statistics Core through the ADRC database; with the Education/Information Transfer Core through monthly clinicopathological conferences and other educational activities. NPMG Core accomplished its specific aims in the past funding period and has added innovative new technologies such as the "molecular autopsy" approach involving routine use of microarray techniques to study gene and protein expression in appropriate autopsy cases.