CORE--NEUROPATHOLOGY AND MOLECULAR GENETICS

核心--神经病理学和分子遗传学

• 批准号: 6798011
• 负责人: HARRY V. VINTERS
• 金额: $ 18.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6798011
• 关键词: Alzheimer' s disease; DNA; biomedical facility; body fluids; cryopreservation; dementia; gene expression; human genetic material tag; human tissue; immunocytochemistry; microarray technology; molecular genetics; neuropathology; postmortem; protein quantitation /detection; tissue /cell preparation; tissue resource /registry

The newly reconfigured Neuropathology and Molecular Genetics (NPMG) Core of the UCLA AIzheimer's Disease Research Center (ADRC) will perform autopsies and genetic studies on patients with neurodegenerative diseases (and appropriate controls) and will serve as a tissue/fluid and DNA resource for individuals performing innovative research on Alzheimer's disease (AD) and non-AD dementias. The specific aims of the Core are to carry out comprehensive postmortem examinations; harvest and store relevant tissues and fluids; assess lesions semi-quantitatively using special stains and immunohistochemistry; provide definitive diagnoses; prepare tissue microarrays for protein expression studies; expand the genetic resource to include apolipoprotein E (apoE) determinations, DNA storage, and preparation of cDNA on patients with known or suspected mutations; and support other Cores and Projects. Project 2 will use brain tissue from the NPMG Core. The autopsy and tissue harvesting/sampling method has evolved and been optimized in conjunction with interactions with the National Alzheimer's Coordinating Center (NACC). Genetic and autopsy information will be maintained in the ADRC database and transmitted to the ARCC database. The Core has performed comprehensive autopsies on 98 patients characterized in Clinical Core; and supplies tissue to investigators locally and nationally. NPMG Core interacts with Clinical Core to provide autopsy confirmation of clinical diagnoses; with Imaging Core to supply autopsy confirmed diagnoses with patients studied in neuroimaging; with the Data Management and Statistics Core through the ADRC database; with the Education/Information Transfer Core through monthly clinicopathological conferences and other educational activities. NPMG Core accomplished its specific aims in the past funding period and has added innovative new technologies such as the "molecular autopsy" approach involving routine use of microarray techniques to study gene and protein expression in appropriate autopsy cases.

加州大学洛杉矶分校新近重新配置的神经病理学和分子遗传学 (NPMG) 核心 艾茨海默病研究中心 (ADRC) 将对神经退行性疾病患者（以及适当的对照）进行尸检和基因研究，并将为对阿尔茨海默病 (AD) 和非 AD 痴呆症进行创新研究的个人提供组织/液体和 DNA 资源。核心的具体目标是进行全面的尸检；收获并储存相关组织和液体；使用特殊染色和免疫组织化学半定量评估病变；提供明确的诊断；制备用于蛋白质表达研究的组织微阵列；扩大遗传资源，包括载脂蛋白 E (apoE) 测定、DNA 储存以及已知或疑似突变患者的 cDNA 制备；并支持其他核心和项目。项目 2 将使用 NPMG 核心的脑组织。尸检和组织采集/采样方法在与国家阿尔茨海默病协调中心 (NACC) 的互动中不断发展和优化。将保留遗传和尸检信息 存入 ADRC 数据库并传输至 ARCC 数据库。核心已执行 对临床核心中描述的 98 名患者进行全面尸检；并提供组织 当地和全国的调查人员。 NPMG Core 与 Clinical Core 互动以提供尸检 临床诊断的确认；使用 Imaging Core 为神经影像学研究的患者提供尸检确诊结果；通过 ADRC 数据库使用数据管理和统计核心；通过每月的临床病理学会议和其他教育活动与教育/信息传输核心合作。 NPMG Core 在过去的资助期间实现了其特定目标，并增加了创新的新技术，例如“分子尸检”方法，涉及常规使用微阵列技术来研究适当尸检病例中的基因和蛋白质表达。