CORE--NEUROPATHOLOGY AND MOLECULAR GENETICS

核心--神经病理学和分子遗传学

• 批准号: 6798011
• 负责人: HARRY V. VINTERS
• 金额: $ 18.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6798011
• 关键词: Alzheimer' s disease; DNA; biomedical facility; body fluids; cryopreservation; dementia; gene expression; human genetic material tag; human tissue; immunocytochemistry; microarray technology; molecular genetics; neuropathology; postmortem; protein quantitation /detection; tissue /cell preparation; tissue resource /registry

The newly reconfigured Neuropathology and Molecular Genetics (NPMG) Core of the UCLA AIzheimer's Disease Research Center (ADRC) will perform autopsies and genetic studies on patients with neurodegenerative diseases (and appropriate controls) and will serve as a tissue/fluid and DNA resource for individuals performing innovative research on Alzheimer's disease (AD) and non-AD dementias. The specific aims of the Core are to carry out comprehensive postmortem examinations; harvest and store relevant tissues and fluids; assess lesions semi-quantitatively using special stains and immunohistochemistry; provide definitive diagnoses; prepare tissue microarrays for protein expression studies; expand the genetic resource to include apolipoprotein E (apoE) determinations, DNA storage, and preparation of cDNA on patients with known or suspected mutations; and support other Cores and Projects. Project 2 will use brain tissue from the NPMG Core. The autopsy and tissue harvesting/sampling method has evolved and been optimized in conjunction with interactions with the National Alzheimer's Coordinating Center (NACC). Genetic and autopsy information will be maintained in the ADRC database and transmitted to the ARCC database. The Core has performed comprehensive autopsies on 98 patients characterized in Clinical Core; and supplies tissue to investigators locally and nationally. NPMG Core interacts with Clinical Core to provide autopsy confirmation of clinical diagnoses; with Imaging Core to supply autopsy confirmed diagnoses with patients studied in neuroimaging; with the Data Management and Statistics Core through the ADRC database; with the Education/Information Transfer Core through monthly clinicopathological conferences and other educational activities. NPMG Core accomplished its specific aims in the past funding period and has added innovative new technologies such as the "molecular autopsy" approach involving routine use of microarray techniques to study gene and protein expression in appropriate autopsy cases.

加州大学洛杉矶分校新重组的神经病理和分子遗传学(NPMG)核心 艾兹海默病研究中心(ADRC)将对神经退行性疾病患者(和适当的对照)进行尸检和基因研究，并将为对阿尔茨海默病(AD)和非AD痴呆进行创新研究的个人提供组织/液体和DNA资源。该中心的具体目标是进行全面的尸检；采集和储存相关组织和液体；使用特殊染色和免疫组织化学对病变进行半定量评估；提供明确的诊断；为蛋白质表达研究准备组织微阵列；扩大遗传资源，包括载脂蛋白E(ApoE)测定、DNA储存和对已知或可疑突变患者的cDNA制备；以及支持其他核心和项目。项目2将使用NPMG核心的脑组织。尸检和组织采集/采样方法已经演变，并结合与国家阿尔茨海默氏症协调中心(NACC)的互动进行了优化。遗传和尸检信息将被保留 在ADRC数据库中，并传输到ARCC数据库。核心已经完成了 对98名具有临床核心特征的患者进行了全面尸检；并为 当地和全国的调查人员。NPMG Core与临床Core互动提供尸检 与数据管理和统计核心通过ADRC数据库；与教育/信息传输核心通过每月临床病理会议和其他教育活动，与教育/信息传输核心合作。NPMG Core在过去的资助期间实现了其具体目标，并增加了创新的新技术，例如“分子尸检”方法，该方法涉及常规使用微阵列技术来研究适当尸检案例中的基因和蛋白质表达。