HEALTH EFFECTS AND BIODEGRADATION OF COMPLEX MIXTURE

总体描述 这是一个多学科的调查机制的不利 暴露于致癌金属的复杂混合物和 多环芳烃（PAHs），以及开发有效的 金属污染混合物中多环芳烃的生物修复策略。四 生物医学研究项目涉及毒性机制， 致癌金属的致突变性及其对PAH代谢的影响， 致突变性两个项目将利用酵母、哺乳动物 细胞和转基因小鼠，以阐明铬和 砷及其对多环芳烃致突变性的影响。一个项目的重点是 确定铬和砷对AH受体依赖性多环芳烃的影响 新陈代谢.一个项目将调查砷对信号的影响， 在人皮肤黑素细胞和角质形成细胞中的转导事件。最终 这项生物医学研究的目标是通过一个更好的方法来改善风险评估。 完全理解毒性和致突变性的潜在机制。 四个非生物医学研究项目的重点是发展 多环芳烃的生物降解策略和金属对 微生物种群的生存力和降解能力。另一个项目将 评估土壤生物膜中微生物的降解能力。一个项目 将集中在降解微生物种群的表征和 遗传学是其生物降解能力和对金属反应的基础。 其中一个项目将调查根际对微生物的影响， 社区及其退化能力。 一个项目将侧重于 生物化学和遗传学的多环芳烃生物降解的复杂混合物，或 多环芳烃和金属这种非生物医学研究的最终目标是 制定有效和高效的生物修复策略。的 分析核心和生物统计核心提供基本服务， 为项目制定方法，从而采取更加综合的办法。 其他计划的目标是提供一个程序和地方之间的联系， 区域环境卫生专业人员和科学/卫生教育 专业人员，并提供一个跨学科的博士培训计划。 环境健康科学专业的学生和博士后助理。的 长期目标是促进旨在应用新的 复杂混合物对人体健康潜在影响的机理信息 生物修复策略的开发和评估， 从而减少风险。

OVERALL DESCRIPTION This is a multidisciplinary investigation of mechanisms underlying the adverse health effects of exposure to complex mixtures of carcinogenic metals and polycyclic aromatic hydrocarbons (PAHs), and the development of effective strategies for bioremediation of PAHs in metal-contaminated mixtures. The four biomedical research projects address the mechanisms of toxicity and mutagenicity of carcinogenic metals and their impact on PAH metabolism and mutagenicity. Two projects will make use of assay systems in yeast, mammalian cell, and transgenic mouse to elucidate the mutagenicity of chromium and arsenic, and their impact on the mutagenicity of PAHs. One project is focused on determining the effect of chromium and arsenic on Ah receptor-dependent PAH metabolism. One project will investigate the impact of arsenic on signal transduction events in human skin melanocytes and karatinocytes. The ultimate goal of this biomedical research is to improve risk assessment through a more complete understanding of underlying mechanisms of toxicity and mutagenicity. The four non-biomedical research projects focus on the development of strategies for the biodegradation of PAHs and on the impact of metals on the viability and degradative capacity of microbial populations. Another project will assess the degradative capacity of microorganisms in soil biofilms. One project will focuse on a characterization of degradative microbial populations and the genetics underlying their biodegradation capacity and responses to metals. One project will investigate the influence of the rhizosphere on microbial communities and their degradative capacity. One project will focuse on the biochemistry and genetics of PAH biodegradation in complex mixtures of PAHs or PAHs and metals. The ultimate goal of this non-biomedical research is the development of effective and efficient bioremediation strategies. The Analytical Core and the Biostatistics Core provide essential services and methods development for the projects, leading to a more integrated approach. Other program goals are to provide a link between the program and local and regional environmental health professionals and science/health education professionals, and to provide an interdisciplinary training program for Ph.D. students and postdoctoral assistants in environmental health sciences. The long term goal is to promote collaborative efforts aimed at applying the new mechanistic information on potential health effects of complex mixtures to the development and evaluation of bioremediation strategies which will ultimately lead to risk reduction.