HEALTH EFFECTS AND BIODEGRADATION OF COMPLEX MIXTURE

总体描述 这是一项多学科的研究，目的是为了更好地研究 接触致癌金属和致癌金属的复杂混合物对健康的影响 多环芳烃(PAHs)及其高效开发 金属污染混合物中多环芳烃的生物修复策略。四个 生物医学研究项目解决了毒性和 致癌金属的致突变性及其对多环芳烃代谢的影响 致突变性。两个项目将利用酵母、哺乳动物的检测系统 细胞和转基因小鼠，以阐明铬和 砷及其对多环芳烃致突变性的影响。一个项目的重点是 铬和砷对AH受体依赖性多环芳烃的影响 新陈代谢。其中一个项目将调查砷对信号的影响 人类皮肤黑素细胞和核基质细胞中的转导事件。终极的 这项生物医学研究的目标是通过更多的 完全了解潜在的毒性和致突变性机制。 四个非生物医学研究项目的重点是开发 多环芳烃的生物降解战略和金属对环境的影响 微生物种群的生存能力和降解能力。另一个项目将 评估土壤生物膜中微生物的降解能力。一个项目 将集中于降解微生物种群的特征和 它们的生物降解能力和对金属的反应背后的遗传学。 其中一个项目将调查根际对微生物的影响。 群落及其退化能力。其中一个项目将专注于 多环芳烃生物降解的生物化学和遗传学研究 多环芳烃和金属。这项非生物医学研究的最终目标是 制定有效和高效的生物修复战略。这个 分析核心和生物统计核心提供基本服务和 项目的方法开发，导致更综合的方法。 其他计划目标是在计划与当地和 区域环境卫生专业人员和科学/健康教育 专业人员，并为博士提供跨学科培训计划。 环境健康科学专业的学生和博士后助理。这个 长期目标是促进旨在应用新的 关于复杂混合物对人体潜在健康影响的机理信息 制定和评估生物修复战略，最终将 导致风险降低。

OVERALL DESCRIPTION This is a multidisciplinary investigation of mechanisms underlying the adverse health effects of exposure to complex mixtures of carcinogenic metals and polycyclic aromatic hydrocarbons (PAHs), and the development of effective strategies for bioremediation of PAHs in metal-contaminated mixtures. The four biomedical research projects address the mechanisms of toxicity and mutagenicity of carcinogenic metals and their impact on PAH metabolism and mutagenicity. Two projects will make use of assay systems in yeast, mammalian cell, and transgenic mouse to elucidate the mutagenicity of chromium and arsenic, and their impact on the mutagenicity of PAHs. One project is focused on determining the effect of chromium and arsenic on Ah receptor-dependent PAH metabolism. One project will investigate the impact of arsenic on signal transduction events in human skin melanocytes and karatinocytes. The ultimate goal of this biomedical research is to improve risk assessment through a more complete understanding of underlying mechanisms of toxicity and mutagenicity. The four non-biomedical research projects focus on the development of strategies for the biodegradation of PAHs and on the impact of metals on the viability and degradative capacity of microbial populations. Another project will assess the degradative capacity of microorganisms in soil biofilms. One project will focuse on a characterization of degradative microbial populations and the genetics underlying their biodegradation capacity and responses to metals. One project will investigate the influence of the rhizosphere on microbial communities and their degradative capacity. One project will focuse on the biochemistry and genetics of PAH biodegradation in complex mixtures of PAHs or PAHs and metals. The ultimate goal of this non-biomedical research is the development of effective and efficient bioremediation strategies. The Analytical Core and the Biostatistics Core provide essential services and methods development for the projects, leading to a more integrated approach. Other program goals are to provide a link between the program and local and regional environmental health professionals and science/health education professionals, and to provide an interdisciplinary training program for Ph.D. students and postdoctoral assistants in environmental health sciences. The long term goal is to promote collaborative efforts aimed at applying the new mechanistic information on potential health effects of complex mixtures to the development and evaluation of bioremediation strategies which will ultimately lead to risk reduction.