A FACILITY FOR S. POMBE MICROARRAYS

描述(申请人提供)：裂变的基因组序列 酵母菌S.pombe即将问世。这种酵母提供了一种强大的基因 和分子体系，这是目前研究的主要焦点约40 美国的实验室，欧洲和日本的实验室数量更多。许多 包括珍妮特·莱瑟伍德博士在内的美国实验室 和A.Bruce Futcher，研究细胞周期，S.pombe是一个 优秀的模式生物。斑点基因芯片是一种廉价、快速、 一种从细胞中收集数据和调查多主题的高效方法 生理和转录电路到染色体结构，信使核糖核酸剪接， 翻译调控、DNA复制控制和减数分裂重组。 在这里，建议建立一个生产和使用S。 庞贝cDNA微阵列。这些微阵列将可供社区使用 在美国的S.pombe工人，以及，如果资源允许，到其他地方的工人 国家。微阵列将在纽约州立大学石溪分校的一个设施中制造。 该设施的外部用户将进行为微阵列设计的实验 分析，分离RNA(或DNA)，验证样本，并将RNA转换为 标记c DNA或标记分离的DNA。这个标记的DNA将被发送到 设施，在那里它将与微阵列杂交，扫描，并 遵循标准的分析形式。结果将发布在网络上 供用户进一步分析的站点。用于更广泛数据的资源 分析将在S的网站上提供。

DESCRIPTION (provided by applicant): The genomic sequence of the fission yeast S. pombe will soon be published. This yeast provides a powerful genetic and molecular system, which is currently the major focus of study for about 40 laboratories in the U.S., and an even larger number in Europe and Japan. Many of the laboratories in the U.S., including those of Drs. Janet Leatherwood and A. Bruce Futcher, work on the cell cycle, for which S. pombe is an excellent model organism. Spotted cDNA microarrays are a cheap, rapid, efficient method for collecting data and investigating many subjects from cell physiology and transcription circuits to chromosome structure, mRNA splicing, translational regulation, DNA replication control, and meiotic recombination. Here, it is proposed to create a facility for the production and use of S. pombe cDNA microarrays. These microarrays would be available to the community of S. pombe workers in the U.S., and, if resources permit, to workers in other countries. Microarrays would be made at a facility at the SUNY, Stony Brook. Outside users of the facility would do experiments designed for microarray analysis, isolate RNA (or DNA), validate the samples, and convert the RNA to labeled cDNA or label the isolated DNA. This labeled DNA would be sent to the facility, where it would be hybridized to the microarrays, scanned, and subjected to standard forms of analysis. Results would be posted to a Web site for further analysis by the user. Resources for more extensive data analysis would be available on the facility?s Web site.