A FACILITY FOR S. POMBE MICROARRAYS

粟酒裂殖酵母微阵列设备

• 批准号: 6771209
• 负责人: JANET K LEATHERWOOD
• 金额: $ 20.85万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-16 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6771209
• 关键词: Schizosaccharomyces pombe; biomedical facility; complementary DNA; microarray technology

DESCRIPTION (provided by applicant): The genomic sequence of the fission yeast S. pombe will soon be published. This yeast provides a powerful genetic and molecular system, which is currently the major focus of study for about 40 laboratories in the U.S., and an even larger number in Europe and Japan. Many of the laboratories in the U.S., including those of Drs. Janet Leatherwood and A. Bruce Futcher, work on the cell cycle, for which S. pombe is an excellent model organism. Spotted cDNA microarrays are a cheap, rapid, efficient method for collecting data and investigating many subjects from cell physiology and transcription circuits to chromosome structure, mRNA splicing, translational regulation, DNA replication control, and meiotic recombination. Here, it is proposed to create a facility for the production and use of S. pombe cDNA microarrays. These microarrays would be available to the community of S. pombe workers in the U.S., and, if resources permit, to workers in other countries. Microarrays would be made at a facility at the SUNY, Stony Brook. Outside users of the facility would do experiments designed for microarray analysis, isolate RNA (or DNA), validate the samples, and convert the RNA to labeled cDNA or label the isolated DNA. This labeled DNA would be sent to the facility, where it would be hybridized to the microarrays, scanned, and subjected to standard forms of analysis. Results would be posted to a Web site for further analysis by the user. Resources for more extensive data analysis would be available on the facility?s Web site.

描述（由申请人提供）： 酵母酿酒Pombe将很快出版。这种酵母提供了强大的遗传 和分子系统，这是目前研究的主要焦点约40 美国的实验室，在欧洲和日本的数量甚至更多。许多 在美国的实验室里，包括珍妮特·莱瑟伍德博士 和A.布鲁斯福彻，工作的细胞周期，其中S。庞贝是一个 优秀的模式生物。斑点cDNA微阵列是一种廉价、快速、 从细胞中收集数据和研究多个主题的有效方法 生理学和转录电路到染色体结构，mRNA剪接， 翻译调控、DNA复制控制和减数分裂重组。 这里，建议建立一个生产和使用S的设施。 粟酒cDNA微阵列这些微阵列将提供给社区 色葡萄美国的粟酒工人，在资源允许的情况下， 国家微阵列将在纽约州立大学斯托尼布鲁克的一个设施中制造。 该设施的外部用户将做专为微阵列设计的实验 分析，分离RNA（或DNA），验证样品，并将RNA转化为 标记的cDNA或标记分离的DNA。这个标记的DNA会被送到 设施，在那里它将杂交到微阵列，扫描， 进行标准形式的分析。结果会被发布到网上 供用户进一步分析。更多数据的资源 分析将在设施上可用？的网站。