Transcription circuitry of the S. pombe cell cycle

描述(申请人提供)：应用微阵列技术通过细胞周期检测庞氏链霉菌(S.pombe)的基因表达，鉴定出750个细胞周期调控基因。这些被放在一簇簇中。对主要簇进行了转录因子的初步指定。在这里，我们将探索S.pombe细胞周期转录网络的电路。我们将定义在有丝分裂中表达的每个主要基因簇是如何受到控制的，以及这些基因簇是如何相互协调的。我们将了解四种主要的细胞周期转录因子MBF、Forkhead、Prz1和ACE2是如何工作的，以及它们是如何受到控制的。我们将开始研究这些转录因子的组合控制。相关性：S.pombe是这里提出的那种全面和全球分析的极佳选择。它一直是并将继续是研究真核细胞周期的主要工具，因此将为理解人类细胞周期调控以及该程序在疾病和肿瘤发生中如何改变提供进一步的见解。

DESCRIPTION (provided by applicant): Microarrays were used to measure gene expression through the cell cycle of S. pombe (fission yeast), and 750 cell cycle regulated genes were identified. These were placed into clusters. Tentative assignments of transcription factors were made to the major clusters. Here, we will explore the circuitry of the S. pombe cell cycle transcriptional network. We will define how each major cluster of genes expressed in mitosis is controlled, and how the clusters are co-ordinated with one another. We will find out how four major cell cycle transcription factors, MBF, Forkhead, Prz1, and Ace2, work, and how they are controlled. We will begin to examine combinatorial control by these transcription factors. Relevance: S. pombe is an excellent choice for the kind of comprehensive and global analysis proposed here. It has been and continues to be a major tool for study of the eukaryotic cell cycle and therefore will provide further insights for understanding human cell cycle regulation and how that program is altered in disease and oncogenesis.