Novel Mechanisms of Gene Regulation in Meiosis: Antisense RNAs and RNA Processing

裂殖酵母S. pombe，与S.酿酒酵母具有高度调节的减数分裂基因表达程序。 然而，似乎减数分裂调控的重要部分不是在转录水平上，而是在RNA加工水平上的转录后。S.粟酒裂殖酵母具有复杂的RNA加工装置，包括剪接调节和RNAi。在这里，我们将集中在这些RNA介导的减数分裂调控机制，以补充研究转录因子为基础的调控在S。啤酒。我们将提供减数分裂转录组的详细特征。我们将研究3'端加工的相互关联的影响（例如，切割和聚腺苷酸化）、RNA周转和RNA剪接，以及这些过程和Mmil蛋白如何结合联合收割机以表达早期减数分裂基因。我们将研究在减数分裂中期发现的许多基因的反义转录本的作用。最后，在Futcher、Neiman和Sternglanz博士的合作下，我们将鉴定出一些肿瘤的RNA靶点。 在两个S. pombe和S.啤酒。

The fission yeast S. pombe, like S. cerevisiae, has a highly regulated gene expression program for meiosis. However, it appears that a significant portion of the meiotic regulation is not at the transcriptional level, but rather post-transcriptional, at the level of RNA processing. S. pombe has a sophisticated apparatus for RNA processing, including regulation of splicing, and including RNAi. Here, we will focus on these RNA-mediated mechanisms of meiotic regulation, to complement investigations of transcription-factor based regulation in S. cerevisiae. We will provide a detailed characterization of the meiotic transcriptome. We will investigate the inter-related effects of 3' end processing (e.g., cleavage and polyadenylation), RNA turnover, and RNA splicing, and how these processes and the Mmil protein combine to express the early meiotic genes. We will investigate the roles of the anti-sense transcripts found over many of the middle meiotic genes. Finally, in collaboration with Drs. Futcher, Neiman, and Sternglanz we will identify the RNA targets of a number of important meiotic RNA binding proteins in both S. pombe and S. cerevisiae.