Comparison of Exhaled and Serum Nitric Oxide Levels

呼出气和血清一氧化氮水平的比较

• 批准号: 7056530
• 负责人: JiYeon Choi
• 金额: $ 4.4万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7056530
• 关键词: artificial respiration; clinical research; human subject; laryngectomy; longitudinal human study; luminescence; nitric oxide; nitrites; nursing research; predoctoral investigator; pulmonary respiration; serum; statistics /biometry

DESCRIPTION (provided by applicant): There is a growing interest in exhaled nitric oxide (NO) as a non-invasive marker of pulmonary inflammation and oxidative stress. Previous studies has focused on use of exhaled NO to diagnose and monitor the airway inflammation in spontaneously breathing patients. However, few studies have involved mechanically ventilated (MV) patients. Airway inflammation and oxidative stress during MV tend to predispose to more serious complications, such as acute lung injury. Thus exhaled NO analysis may also have a potential use in MV patients. As an initial step, it is necessary to explore changes in levels of exhaled NO and its serum marker (serum nitrite [sNO2- ]) over time in patients who require an artificial airway (which disrupts normal NO flow from the nose to the lower airways) and MV (which introduces confounding factors). The purpose of this study is to examine changes in exhaled NO from the nose (nNO), exhaled NO from the lower airways (LNO) and sNO2- over time in: 1) spontaneously breathing patients undergoing tracheostomy or laryngectomy (T/L), and 2) patients undergoing tracheostomy while on MV (T-MV). A prospective, 2x3 repeated measures design will be used. Comparisons will be made between nNO, LNO and sNO2- obtained 1) pre-T/L, Day 1-3 post-T/L, and 1 week after in T/L patients, and 2) pre-tracheostomy, Day 1-3 post-tracheostomy, and 1 week after in T-MV patients. Statistical analysis will include descriptive statistics, 2x3 repeated measures MANCOVA, 1 way repeated measures ANOVA and Sperman's rank correlation.

描述（由申请人提供）：人们越来越关注呼出的一氧化氮（NO）作为肺部炎症和氧化应激的非侵入性标志物。以往的研究主要集中在使用呼出的NO来诊断和监测自主呼吸患者的气道炎症。然而，很少有研究涉及机械通气（MV）患者。MV期间的气道炎症和氧化应激倾向于诱发更严重的并发症，如急性肺损伤。因此，呼出NO分析也可能在MV患者中具有潜在用途。作为第一步，有必要探索需要人工气道（干扰从鼻子到下呼吸道的正常NO流量）和MV（引入混杂因素）的患者呼出NO及其血清标志物（血清亚硝酸盐[sNO 2- ]）水平随时间的变化。本研究的目的是检查从鼻子呼出的NO（nNO），从下气道呼出的NO（LNO）和sNO 2-随时间的变化：1）进行气管切开术或喉切除术（T/L）的自主呼吸患者，2）进行气管切开术，而MV（T-MV）的患者。将使用前瞻性2x 3重复测量设计。将比较1）T/L患者T/L前、T/L后第1-3天和1周后获得的nNO、LNO和sNO 2，以及2）T-MV患者气管切开术前、气管切开术后第1-3天和1周后获得的nNO、LNO和sNO 2。统计分析将包括描述性统计、2x 3重复测量MANCOVA、单因素重复测量ANOVA和Sperman等级相关。